Hormonal regulation and molecular mechanisms of insect host-symbiont crosstalk

2010 Fiscal Year Final Research Report

• Project/Area Number: 21880056
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: FUTAHASHI Ryo National Institute of Advanced Industrial Science and Technology, 生物プロセス研究部門, 研究員 (50549889)
• Project Period (FY): 2009 – 2010
• Keywords: 共生細菌 / 絶食 / 昆虫ホルモン / EST / 体色

Research Abstract

I found that starvation induced by molting hormone is the main cause of reduction of the amount of symbiotic bacteria in hemipteran insects. Also I succeeded in obtaining many novel genes involved in insect symbiosis by the construction of EST database and quantitative expression analysis. Moreover, I established RNAi method for stinkbug insects. Identification of novel symbiosis-associated genes and establishment of functional analysis will allow significant advance in analysis of the molecular mechanisms of host-symbiont crosstalk.

Research Products

• [Journal Article] Laccase2 is required for cuticular pigmentation in stinkbugs. (2011)
  • Author(s): Futahashi R., Tanaka K., Matsuura Y., Tanahashi M., Kikuchi Y., Fukatsu T.
  • Journal Title: Insect Biochemistry and Molecular Biology. 41巻 Pages: 191-196
  • Peer Reviewed
• [Journal Article] RNA interference in Lepidoptera : an overview of successful and unsuccessful studies and implications for experimental design. (2011)
  • Author(s): Terenius O., ほか(23番目の著者 Futahashi R.).
  • Journal Title: Journal of Insect Physiology 57巻 Pages: 231-245
  • Peer Reviewed
• [Journal Article] Repression of tyrosine hydroxylase is responsible for the sex-linked chocolate mutation of the silkworm., Bombyx mori. (2010)
  • Author(s): Liu C., Yamamoto K., Cheng T.C., Kadono-Okuda K., Narukawa J., Liu S.P., Han Y., Futahashi R., Kidokoro K., Noda H.,ほか.
  • Journal Title: Proc Natl Acad Sci USA 107巻 Pages: 12980-12985
  • Peer Reviewed
• [Journal Article] Species-specific coordinated gene expression and trans-regulation of larval color pattern in three swallowtail butterflies. (2010)
  • Author(s): Shirataki H., Futahashi R., Fujiwara H.
  • Journal Title: Evolution and Development 12巻 Pages: 305-314
  • Peer Reviewed
• [Journal Article] Caterpillar color patterns are determined by a two-phase melanin gene pre-patterning process : new evidence from tan and laccase2. (2010)
  • Author(s): Futahashi R., Banno Y., Fujiwara, H.
  • Journal Title: Evolution and Development 12巻 Pages: 157-167
  • Peer Reviewed
• [Presentation] Development and evolution of color pattern diversity in dragonflies. (2011)
  • Author(s): Futahashi R., Fukatsu T.
  • Organizer: 第20回理研CDB会議
  • Place of Presentation: 神戸
  • Year and Date: 2011-02-23
• [Presentation] EST-based screening for genes involved in host-symbiont interactions in the bean bug Riptortus pedestris. (2010)
  • Author(s): Futahashi R., Tanaka K., Tanahashi. M., Kikuchi Y., Fukatsu T.
  • Organizer: 国際生物学賞記念シンポジウム
  • Place of Presentation: つくば
  • Year and Date: 2010-12-07
• [Presentation] Color-pattern evolution and speciation in dragonflies. (2010)
  • Author(s): Futahashi R.
  • Organizer: カイコゲノム関連研究集会
  • Place of Presentation: つくば
  • Year and Date: 2010-11-10
• [Presentation] Comparison of color pattern evolution between dragonflies & butterflies. (2010)
  • Author(s): Futahashi R.
  • Organizer: 第6回国際蝶類学会
  • Place of Presentation: エドモントン(カナダ)
  • Year and Date: 2010-06-30
• [Book] 生物学辞典 (2010)
  • Author(s): 石川統編(二橋亮, 分担執筆)
  • Total Pages: 1616
  • Publisher: 東京化学同人
• [Book] 分子昆虫学 (2009)
  • Author(s): 二橋亮(分担執筆)
  • Total Pages: 448
  • Publisher: 共立出版
• [Remarks] ホームページ等
  • URL: http://staff.aist.go.jp/t-fukatsu/Futahashi_right.html