Analysis of X11 proteins functions in vivo

2010 Fiscal Year Final Research Report

• Project/Area Number: 21890002
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: SAITO Yuhki Hokkaido University, 大学院・薬学研究院, 助教 (70548180)
• Project Period (FY): 2009 – 2010
• Keywords: アルツハイマー病 / てんかん / X11

Research Abstract

X11/X11L doubly-deficient mice suffered from spontaneous epileptic seizures and hyperpolarization-activated cyclic nucleotide gated (HCN) channels function were reduced in X11/X11L doubly-deficient mice brain. The number of amyloid deposits in X11L-KO/APP23 Tg mice brains were significantly increased. The functional domains of X11 proteins to suppress the metabolism of amyloid precursore protein (APP) were carboxy-terminal PDZ domains.

Research Products

• [Journal Article] Increased amyloidogenic processing of transgenic human APP in X11-like deficient mouse brain. (2010)
  • Author(s): Maho Kondo, Maki Shiono, Genzo Ito, Norio Takei, Takahide Matsushima, Masahiro Maeda, Hidenori Taru, Saori Hata, Tohru Yamamoto, Yuhki Saito, Toshiharu Suzuki
  • Journal Title: Mol.Neurodegener. 5 Pages: 35
  • Peer Reviewed
• [Journal Article] Suppression of APP-containing vesicle trafficking and production of β-amyloid by AID/DHHC-12 protein. (2010)
  • Author(s): Chie Mizumaru, Yuhki Saito, Takao Ishikawa, Tomohiro Yoshida, Tohru Yamamoto, Tadashi Nakaya, Toshiharu Suzuki
  • Journal Title: J.Neurochem. 111 Pages: 1213-24
  • Peer Reviewed
• [Presentation] アミロイド前駆体蛋白質(APP)結合分子X11 proteins欠損による痙攣発作発症の分子機構解明 (2010)
  • Author(s): 齋藤有紀、井上剛、鈴木利治
  • Organizer: 第29回日本認知症学会学術集会(21-18)
  • Place of Presentation: ウィンク愛知(愛知県)(ポスター)
  • Year and Date: 20101105-20101106
• [Presentation] A Novel secretory pathway of amyloid precursor protein mediated by X11-like (2010)
  • Author(s): Yuhki Saito, Toshiharu Suzuki
  • Organizer: 50th The American society for cell biology annual meeting(PA 2572)
  • Place of Presentation: Philadelphia(Poster)
  • Year and Date: 2010-12-14
• [Presentation] Functional region of X11-like in the regulation of APP intracellular metabolism and trafficking (2010)
  • Author(s): Maki Shiono, Yuhki Saito, Toshiharu Suzuki
  • Organizer: 50th The American society for cell biology annual meeting(PA 2574)
  • Place of Presentation: Philadelphia(Poster)
  • Year and Date: 2010-12-14
• [Presentation] Neuronal Activity and APP Metabolism in The Brain of X11/X11L Doubly-Deficinet mice (2010)
  • Author(s): Yuhki Saito, Tsuyoshi Inoue, Toshiharu Suzuki
  • Organizer: Alzheimer's Association International Conference on Alzheimer's Disease 2010 (ICAD2010)(P1-112)
  • Place of Presentation: Honolulu, Hawaii(Poster)
  • Year and Date: 2010-07-11
• [Presentation] Expression and localization of Alcadein Along with X11L and APP in mouse brain (2010)
  • Author(s): Yi Piao, Naoya Gotoh, Maho Kondo, Yuhki Saito, Saori Hata, Tohru Yamamoto, Toshiharu Suzuki
  • Organizer: Alzheimer's Association International Conference on Alzheimer's Disease 2010 (ICAD2010)(P1-222)
  • Place of Presentation: Honolulu, Hawaii(Poster)
  • Year and Date: 2010-07-11
• [Presentation] Functional region of X11L in the regulation of APP metabolism and trafficking (2010)
  • Author(s): Maki Shiono, Mayu Akiyama, Yuhki Saito, Toshiharu Suzuki
  • Organizer: Alzheimer's Association International Conference on Alzheimer's Disease 2010 (ICAD 2010)(P1-247)
  • Place of Presentation: Honolulu, Hawaii(Poster)
  • Year and Date: 2010-07-11
• [Presentation] 脳内APP代謝におけるX11-like/X11Lの機能 (2010)
  • Author(s): 齋藤有紀、塩野真紀、鈴木利治
  • Organizer: 第130年会 日本薬学会(一般シンポジウム S22-1)
  • Place of Presentation: ホテルグランヴィア岡山(岡山県)
  • Year and Date: 2010-03-30
• [Presentation] X11 proteins欠損によるAb産生増加と痙攣発作発症の分子基盤解明 (2009)
  • Author(s): 齋藤有紀、井上剛、井本敬二、山本融、鈴木利治
  • Organizer: 第28回日本認知症学会(1-P-3)
  • Place of Presentation: 東北大学百年記念会館(ポスター)
  • Year and Date: 2009-11-20
• [Presentation] X11L欠損マウスにおける脳内human APPのアミロイド産生的代謝 (2009)
  • Author(s): 近藤真帆、佐久間めぐみ、齋藤有紀、伊藤原蔵、前田雅弘、鈴木利治
  • Organizer: 第28回日本認知症学会(1-P-13)
  • Place of Presentation: 東北大学百年記念会館(ポスター)
  • Year and Date: 2009-11-20
• [Presentation] 脳特異的アダプター蛋白質X11およびX11LによるAβ産生抑制の分子機構 (2009)
  • Author(s): 齋藤有紀,近藤真帆,伊藤原蔵,前田雅弘,鈴木利治
  • Organizer: 第82回日本生化学大会(4T9a-4, 4P552)
  • Place of Presentation: 神戸ポートアイランド(口頭&ポスター)
  • Year and Date: 2009-10-24
• [Presentation] APP代謝と細胞内局在に果たすX11Lの機能ドメインの解析 (2009)
  • Author(s): 塩野真希,穐山麻由,山本融,齋藤有紀,鈴木利治
  • Organizer: 第82回日本生化学大会(4P553)
  • Place of Presentation: 神戸ポートアイランド(ポスター)
  • Year and Date: 2009-10-24
• [Presentation] X11Lによるアルツハイマー病原因因子APPの脳内切断制御とアミロイド生成 (2009)
  • Author(s): 近藤真帆,齋藤有紀,前田雅弘,鈴木利治
  • Organizer: 第14回日本病態とプロテアーゼ学会 (J.S.P.P)(G1)
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)(ポスター)
  • Year and Date: 2009-08-21
• [Presentation] Regulation of intracellular APP transport by X11L (2009)
  • Author(s): Maki Shiono, Mayu Akiyama, Yuhki Saito, Tohru Yamamoto, Toshiharu Suzuki
  • Organizer: 12th International Conference on Alzheimer's Disease and Related Disorders (ICAD)(P4-183)
  • Place of Presentation: Vienna, Austria(Poster)
  • Year and Date: 2009-07-15
• [Presentation] Aberrant localization of amyloid precursor protein in X11- and X11L-deficient mice brain (2009)
  • Author(s): Yuhki Saito, Tadashi Nakaya, Toshiharu Suzuki
  • Organizer: 12th International Conference on Alzheimer's Disease and Related Disorders (ICAD)(P1-007)
  • Place of Presentation: Vienna, Austria(Poster)
  • Year and Date: 2009-07-12
• [Presentation] Amyloidgenic Metabolism of human APP in X11-like deficient mice brain (2009)
  • Author(s): Maho Kondo, Megumi Sakuma, Yuhki Saito, Tadashi Nakaya, Masahiro Maeda, Toshiharu Suzuki
  • Organizer: 12th International Conference on Alzheimer's Disease and Related Disorders (ICAD)(P1-158)
  • Place of Presentation: Vienna, Austria(Poster)
  • Year and Date: 2009-07-12
• [Book] Lipids and Cellular Membranes in Amyloid Disease(Edited by Raz Jelinek)(Alzheimer's Disease as a Membrane-Associated Enzymopathy of β-Amyloid Precursor Protein (APP) Secretases) (2011)
  • Author(s): Saori Hata, Yuhki Saito, Toshiharu Suzuki
  • Total Pages: 177-194
  • Publisher: WILEY-VCH
• [Remarks] ホームページ等
  • URL: http://www.pharm.hokudai.ac.jp/shinkei/index.html