2010 Fiscal Year Final Research Report
Network Calcium Imaging in Suprachiasmatic Nucleus
Project/Area Number |
21890003
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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Research Institution | Hokkaido University |
Principal Investigator |
ENOKI Ryosuke Hokkaido University, 大学院・医学研究科, 助教 (00528341)
|
Project Period (FY) |
2009 – 2010
|
Keywords | 視交叉上核 / 概日リズム / カルシウムイメージング / 共焦点顕微鏡 / 神経回路網 |
Research Abstract |
The present study was carried out to visualize the spatiotemporal patterns of neuronal network activities in suprachiasmatic nucleus, the center for mammalian circadian clock. To this goal, I have developed fast scanning single or multi-photon confocal microscopy and methods to load calcium sensitive dye in large neuronal population in suprachiasmatic nucleus. I successfully recorded synaptically-induced or spontaneous calcium concentration changes from hundreds of neurons in deep brain tissue at high spatial (single cell level) and temporal resolutions (millisecond scale).
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