2010 Fiscal Year Final Research Report
Involvement of DNAM-1 in the development of graft-versus-host disease
| Project/Area Number |
21890026
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NABEKURA Tsukasa University of Tsukuba, 大学院・人間総合科学研究科, 研究員 (80550095)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 移植片対宿主病 / GVHD / DNAM-1 / 抗体療法 / 骨髄移植 |
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| Research Abstract |
Graft-versus-host disease (GVHD) is a life-threatening complication following bone marrow transplantation ; however, no effective molecular targeting therapy has been determined. Here, we show that mice that received allogeneic splenocytes deficient in DNAM-1 had significantly lower mortality than those that received wild-type (WT) splenocytes. Donor CD8+T cells deficient in DNAM-1 showed less proliferation and produced less interferon-γ after coculture with allogeneic splenocytes than WT CD8+ T cells. Mice prophylactically treated with an anti-DNAM-1 antibody showed low mortality. Moreover, treatment with a single administration of the antibody after the onset of GVHD prolonged survival. These results indicate that DNAM-1 plays an important role in the development of GVHD and is an ideal molecular target for therapeutic approaches to GVHD.
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