Analysis of the molecular mechanisms controlling self-renewal and differentiation of breast cancer stem cells using systems biological methodology

2010 Fiscal Year Final Research Report

• Project/Area Number: 21890048
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: HINOHARA Kunihiko The University of Tokyo, 医科学研究所, 特任助教 (50549467)
• Project Period (FY): 2009 – 2010
• Keywords: 乳癌 / 癌幹細胞 / GSEA解析 / NF-κB

Research Abstract

We used gene set enrichment analysis (GSEA), which is an analytical method for determining gene expression profiles on the basis of potential pathways and identified that breast cancer cell lines contain a CD44hi/CD24low breast cancer stem cell population with a distinct molecular profile, which includes inflammatory properties. We also identified that NF-κB, one of the main regulator of inflammatory signaling pathway, regulates the self-renewal capacity of breast cancer stem cells.

Research Products

• [Journal Article] Inflammatory signaling pathways in self-renewing breast cancer stem cells. (2010)
  • Author(s): Hinohara K, Gotoh N
  • Journal Title: Curr Opin Pharmacol. 10 Pages: 650-4
  • Peer Reviewed
• [Journal Article] Gene set enrichment analysis provides insight into novel signaling pathways in breast cancer stem cells. (2010)
  • Author(s): Murohashi M., Hinohara K., Kuroda M., Isagawa T., Tsuji S., Kobayashi S., Umezawa K., Tojo A., Aburatani H., Gotoh N.
  • Journal Title: British J.Cancer 102 Pages: 206-212
  • Peer Reviewed
• [Presentation] NF-kappaB regulates the self-renewal of breast cancer stem cells (2010)
  • Author(s): 日野原邦彦、小林誠一郎、梅澤一夫、東條有伸、井上純一郎、後藤典子
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Potential roles of NF-kappa B pathways in breast cancer stem cells (2010)
  • Author(s): 日野原邦彦、室橋道子、砂川孝行、辻真吾、小林誠一郎、梅澤一夫、東條有伸、油谷浩幸、井上純一郎、後藤典子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] Potential roles of NF-κB pathways in breast cancer stem cells (2010)
  • Author(s): 日野原邦彦、室橋道子、黒田雅彦、砂川孝行、辻真吾、小林誠一郎、梅澤一夫、東條有伸、油谷浩幸、後藤典子
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 淡路
  • Year and Date: 2010-05-14
• [Presentation] Potential roles of NF-κB pathways in breast cancer-initiating cells (2010)
  • Author(s): 日野原邦彦、室橋道子、黒田雅彦、砂川孝行、辻真吾、小林誠一郎、梅澤一夫、東條有伸、油谷浩幸、後藤典子
  • Organizer: American Association for Cancer Research 101th Annual Meeting 2010
  • Place of Presentation: Walter E.Washington Convention Center, Washington D.C., USA
  • Year and Date: 2010-04-20
• [Presentation] Potential roles of NF-κB pathways in breast cancer-initiating cells (2009)
  • Author(s): 日野原邦彦、室橋道子、黒田雅彦、砂川孝行、辻真吾、小林誠一郎、梅澤一夫、東條有伸、油谷浩幸、後藤典子
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] ヒト乳癌細胞株における癌幹細胞の分子シグネチャー (2009)
  • Author(s): 室橋道子、日野原邦彦、黒田雅彦、砂川孝行、辻真吾、小林誠一郎、梅澤一夫、東條有伸、油谷浩幸、後藤典子
  • Organizer: 第68回日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Remarks] ホームページ等
  • URL: http://www.ims.u-tokyo.ac.jp/system-seimei/index.htm