Analysis of leukemia stem cell regulation by leukemia oncogene Evi-1

2010 Fiscal Year Final Research Report

• Project/Area Number: 21890055
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Tomohiko The University of Tokyo, 医学部附属病院, 特任臨床医 (90553694)
• Project Period (FY): 2009 – 2010
• Keywords: 癌 / 白血病 / 幹細胞

Research Abstract

We have recently generated Evi-1-GFP knock-in mice, which can trace Evi1 mRNA level in vivo, and we revealed that Evi-1 is highly expressed at both fetal and adult hematopoietic stem cells (HSCs). In addition, we also clarified that Evi-1 positive murine HSC (KSL) function as long-term HSC by bone marrow transplantation assay, and that Evi-1 can mark long-term HSC which is the most immature hematopoietic cells. (These data are now on submission) Next, for the analysis of leukemia stem cell regulation by Evi-1, we made chronic myeloid leukemia (CML) mouse of Evi-1-GFP knockin origin by using BCR-ABL oncogene. We revealed that Evi-1 positive KSL cells had highest Evi-1 expression in these model mice which implied that Evi-1 could regulate CML stem cells. Our original analysis of leukemia stem cell is still ongoing for the purpose of establishing specific therapy of CML stem cells.

Research Products

• [Presentation] Evi1 Is a Stem Cell-Specific Regulator of Self-Renewal Capacity In the Definitive Hematopoietic System (2010)
  • Author(s): Keisuke Kataoka, Tomohiko Sato, Akihide Yoshimi, Susumu Goyama, Takako Tsuruta, Shunya Arai, Yoichi Imai, Katsuyoshi Kumagai, Naoto Kubota, Takashi Kadowaki, Mineo Kurokawa
  • Organizer: 52nd American Society of Hematology Annual Meeting (838)
  • Place of Presentation: Orlando, USA.(Oral presentation)
  • Year and Date: 20101204-20101207
• [Presentation] Evi1 expression marks long-term repopulating hematopoietic stem cells. (2010)
  • Author(s): Keisuke Kataoka, Tomohiko Sato, Akihide Yoshimi, Susumu Goyama, Shunya Arai, Yoichi Imai, Katsuyoshi Kumagai, Naoto Kubota, Takashi Kadowaki, Mineo Kurokawa
  • Organizer: 第72回日本血液学会学術集会(OS-1-1)
  • Place of Presentation: 横浜
  • Year and Date: 20100924-20100926
• [Presentation] Evi1 expression marks long-term repopulating hematopoietic stem cells. (2010)
  • Author(s): Keisuke Kataoka, Tomohiko Sato, Akihide Yoshimi, Susumu Goyama, Shunya Arai, Yoichi Imai, Mineo Kurokawa
  • Organizer: 第69日本癌学会学術総会(O-520)
  • Place of Presentation: 大阪
  • Year and Date: 20100922-20100924
• [Presentation] Evi1 expression marks long-term repopulating hematopoietic stem cells. (2010)
  • Author(s): Keisuke Kataoka, Tomohiko Sato, Akihide Yoshimi, Susumu Goyama, Shunya Arai, Yoichi Imai, Katsuyoshi Kumagai, Naoto Kubota, Takashi Kadowaki, Mineo Kurokawa
  • Organizer: 第8回幹細胞シンポジウム(O-29)
  • Place of Presentation: 淡路島
  • Year and Date: 20100513-20100515
• [Remarks] ホームページ等