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2010 Fiscal Year Final Research Report

The engineering of neuron specific targeted helper-dependent adenovirus vectors for the treatment of neuropathic pain.

Research Project

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Project/Area Number 21890109
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionShiga University of Medical Science

Principal Investigator

TERASHIMA Tomoya  Shiga University of Medical Science, 医学部, 助教 (40378485)

Project Period (FY) 2009 – 2010
Keywordsウイルス / バイオテクノロジー / 再生医学 / 脳・神経 / 遺伝子
Research Abstract

(1) We established two stable cell lines, which were 293-cre cells and 293-fiber cells. 293-cre cells continuously expressed cre recombinase, are essential to be produced helper-dependent adenovirus vectors as one of the gutless virus vectors. 293-fiber cells are used to be produced and amplified the helper virus with high affinities against neuronal cells.
(2) We stored much amount of wild type helper virus with 293-cre cells to be produced wild type HDAd.
(3) We stored much amount of DRG targeting helper virus with 293-fiber cells to be produced DRG targeting HDAd.
(4) cDNA of glutamic acid decarboxylase (GAD) was subcloned from cDNA library of mouse brain and inserted into pdelta28 vector with EF-1 promoter for HDAd production. (We also produced pdelta28-TNF-siRNA vector for knockdown of TNF-alpha.)
(5) We published the article for selective gene therapy for the neurons with tissue specific targeting HDAd system. And we made the presentation about them at severa meeting and propagated the usefulness and the possibilities in future of our studies.

  • Research Products

    (9 results)

All 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] Hyperglycemia induces oxidative stress and impairs axonal transport rates in mice.2010

    • Author(s)
      Sharma R, Buras E, Terashima T, Serrano F, Massaad CA, Hu L, Bitner B, Inoue T, Chan L, Pautler RG.
    • Journal Title

      PLoS One 5

      Pages: e13463

    • Peer Reviewed
  • [Journal Article] DRG-targeted helper-dependent adenoviruses mediate selective gene delivery for therapeutic rescue of sensory neuronopathies in mice.2009

    • Author(s)
      Terashima T, Oka K, Kritz AB, Kojima H, Baker AH, Chan L.
    • Journal Title

      J Clin Invest 119

      Pages: 2100-2112

    • Peer Reviewed
  • [Journal Article] A neuronal VLDLR variant lacking the third complement-type repeat exhibits high capacity binding of apoE containing lipoproteins.2009

    • Author(s)
      Sakai K, Tiebel O, Ljungberg MC, Sullivan M, Lee HJ, Terashima T, Li R, Kobayashi K, Lu HC, Chan L, Oka K.
    • Journal Title

      Brain Res 1276

      Pages: 11-21

    • Peer Reviewed
  • [Presentation] Andrew H Baker and Lawrence Chan. ENGI NEERING DRG-TARGETED HELPER-DEPENDENT A DENOVIRUSES FOR SELECTIVE GENE DELIVERY2010

    • Author(s)
      Tomoya Terashima, Kazuhiro Oka, Angelika B Kritz, Hideto Kojima, Isamu Yamakawa, Hiromichi Kawai, Mitsuru Sanada
    • Organizer
      第16回日本遺伝子治療学会学術集会
    • Place of Presentation
      栃木
    • Year and Date
      20100701-20100702
  • [Presentation] Lawrence Chan DRG-targeted helper-dependent adenovirus vectorによる感覚神経選択的遺伝子治療2010

    • Author(s)
      寺島智也、Kazuhiro Oka、Angelika B.Kritz、小島秀人、山川勇、川合寛道、真田充、片山由理、Andrew H.Baker
    • Organizer
      第51回日本神経学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-05-21
  • [Presentation] Lawrence Chan. マウス後根神経節標的ヘルパー依存型アデノウイルスベクターによる感覚神経細胞障害の選択的遺伝子治療2010

    • Author(s)
      寺島智也、Kazuhiro Oka、Angelika B.Kritz、小島秀人、山川勇、川合寛道、真田充、片山由理、Andrew H.Baker
    • Organizer
      第9回日本再生医療学会総会
    • Place of Presentation
      広島
    • Year and Date
      2010-03-19
  • [Presentation] Lawrence Chan. Engineering DRG-targeted helper-dependent adenoviruses for selective gene delivery.2009

    • Author(s)
      Tomoya Terashima, Kazuhiro Oka, Angelika B Kritz, Hideto Kojima, Andrew H Baker
    • Organizer
      第19回世界神経会議(World Congress of Neurology)
    • Place of Presentation
      タイ王国
    • Year and Date
      2009-10-28
  • [Remarks] 滋賀医科大学ホームページ

    • URL

      http://www.shiga-med.ac.jp/education/newresearch/2009_terashima.html

  • [Remarks] ベイラー医科大学ホームページ

    • URL

      http://www.bcm.edu/news/item.cfm?newsID=1467

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Published: 2012-01-26   Modified: 2016-04-21  

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