2010 Fiscal Year Final Research Report
Assessment of antigen processing and presentation of CD40 activated B cells
| Project/Area Number |
21890147
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Okayama University |
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Principal Investigator |
KONDO Eisei Okayama University, 岡山大学病院, 助教 (30379747)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 腫瘍免疫 / CD40活性化B細胞 / CMV / mRNA transfection |
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| Research Abstract |
It has been demonstrated that CD40-activated B (CD40-B) cells are potent antigen presenting cells that can be expanded significantly ex vivo.
Several methods to antigen load CD40-Bs to generate viral or tumor-antigen specific CD8+ T cells have been identified. However, it is unclear how CD40-Bs take up and process exogenous antigen in an B cell receptor independent manner to stimulate CD4+ T cells. CD4+ T cells specific for multiple epitopes were successfully generated in stimulation with CD40-B cells pulsed with recombinant protein. In addition, CD40-Bs were able to be easily transfected with in vitro transcribed-mRNA. CD40-B cells are thought to be useful in cellular therapy.
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