2010 Fiscal Year Final Research Report
Study on the Mechanisms of Escaping Senescence of Cancer Cells.
| Project/Area Number |
21890183
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | 独立行政法人国立がん研究センター (2010)
Kyushu University (2009) |
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Principal Investigator |
MATSUNOBU Tomoya 独立行政法人国立がん研究センター, 中央病院, 研究員 (20543416)
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| Project Period (FY) |
2009 – 2010
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| Keywords | がん / 細胞老化 |
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| Research Abstract |
Previously, I have reported the possible role of EWS-Fli1 chimeric protein in evasion of senescence in Ewing family tumors. In this study, to elucidate the molecular mechanism of escaping senescence of Ewing sarcoma cells mediated by EWS-Fli1, morphological and microarray analyses were performed. By 24 hours after deletion of EWS-Fli1 by siRNA, Ewing sarcoma cells displayed a flattened and enlarged morphology, which could imply a senescence-like phenotype. Microarray analysis showed that depletion of EWS-Fli1 by siRNA caused dramatic change of the mRNA expression of a large number of genes, including cell-cycle regulatory genes, in a time-dependent manner.
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