2010 Fiscal Year Final Research Report
Examination of novel epitopes and development of inhibitory antibodies for the effective inhibition of HIV infection
| Project/Area Number |
21890196
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TODA Teppei Kumamoto University, エイズ学研究センター, COEリサーチアソシエイト (60551273)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 感染症 / ウイルス / 免疫学 |
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| Research Abstract |
For the examination of novel epitopes that can effectively inhibit HIV infection, we immunized soluble CD4 and membrane fusion intermediate complex of HIV envelope-CD4-chemikine receptor that is formed on the cell membrane of CD4+ T cell during the infection of HIV, to the high-affinity antibody (Ab) producing mice. And we established anti-CD4 monoclonal (m) Ab and anti-fusion complex (FC) mAb libraries. Anti-CD4 R275 mAb recognized novel epitope of domain three of CD4 and inhibited HIV-1 infection. We established anti-FC mAb library that recognized dynamic epitopes appearing during the membrane fusion process. Our mAb library may be useful on the examination of novel target of inhibition of HIV infection.
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