2010 Fiscal Year Final Research Report
Exploitation of tissue specific proton pump inhibitors
| Project/Area Number |
21890239
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Iwate Medical University |
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Principal Investigator |
SEKIYA Mizuki Iwate Medical University, 薬学部, 助教 (70509033)
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| Project Period (FY) |
2009 – 2010
|
| Keywords | 酵素 / プロトンATPase / V-ATPase / ATP合成酵素 / 阻害剤 |
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| Research Abstract |
I analyzed rotation mechanism of F-type ATPase using single molecule observation system in order to understand those of proton ATPases including V-type ATPase in osteoclast. Thermodynamic analysis of the rotation indicated that a specific γ-β interaction in F1 sector is important for flat energy pathway during the rotation, and F1 requires ~2-fold higher activation energy for the transition from the active state (continuous rotation) to the inhibited state (~1 sec pausing) compared with that for normal steady-state rotation during the active state.
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