Elucidation of the periodontal disease using cell cycle-arrested quiescent osteoclast precursors(QOP).

2010 Fiscal Year Final Research Report

• Project/Area Number: 21890274
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: MUTO Akinori Matsumoto Dental University, 歯学部, 助教 (50549433)
• Project Period (FY): 2009 – 2010
• Keywords: 歯周病 / 骨吸収 / 破骨細胞 / ケモカイン / 骨粗鬆症

Research Abstract

In a differentiation process of the osteoclast, We analyzed the character of osteoclast precursor cell (cell cycle-arrested quiescent osteoclast precursors : QOP) which differentiated to osteoclast without cell cycle. In this study, We analyzed an existence style of QOP at the time of the ectopic bone by bone morphogenetic protein-2(BMP) to examine whether QOP existed in blood as well as bone marrow. QOP had a low expression of macrophage marker, but had a high osteoclast marker. In addition, QOP got the views that were near to osteoclast by an electron microscope. Furthermore, QOP appeared around an ectopic bone. Therefore, QOP have a possibility that it is a specific precursor for osteoclast. And QOP which stopped in an existing cell cycle specifically migrates the osteoclast formed to an ectopic bone in the osteoclast formation part, and it is thought that I differentiate to osteoclast by bone resorption stimulation. This concept prints a different concept that decrease of bone resorption factor for inflammatory bone destruction lesions such as periodontal disease and thinks that I make a help to elucidation of new bone disease mechanism.

Research Products

• [Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo. (2010)
  • Author(s): Takahashi N, Muto A, Arai A, Mizoguchi T
  • Journal Title: Adv Exp Med Biol 658 Pages: 21-30
  • Peer Reviewed
• [Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo. (2009)
  • Author(s): Mizoguchi T, Muto A, Udagawa N, Arai A, Yamashita T, Hosoya A, Ninomiya T, Nakamura H, Yamamoto Y, Kinugawa S, Nakamura M, Nakamichi Y, Kobayashi Y, Nagasawa S, Oda K, Tanaka H, Tagaya M, Penninger JM, Ito M, Takahashi N
  • Journal Title: J Cell Biol 184 Pages: 541-554
  • Peer Reviewed
• [Presentation] 歯周基本治療により改善が見られた薬物性歯肉増殖症の2症例 (2010)
  • Author(s): 海瀬聖仁、武藤昭紀、西田英作、佐藤哲夫、吉成伸夫
  • Organizer: 松本歯学会
  • Place of Presentation: 長野
  • Year and Date: 2010-12-12
• [Presentation] 破骨細胞分化に特化した前駆細胞は血流を介して破骨細胞形成部位に遊走する。 (2009)
  • Author(s): 武藤昭紀, 溝口利英, 荒井敦, 小林泰浩, 宇田川信之, 高橋直之, 吉成伸夫
  • Organizer: 第4回日本歯周病学会中部地区大学・日本臨床歯周病学会中部支部合同研究会
  • Place of Presentation: 長野
  • Year and Date: 2009-11-06
• [Presentation] Characterization of cell cycle-arrested quiescent osteoclast precursors (2009)
  • Author(s): Mizoguchi T, Muto A, Udagawa N, Arai A, Kobayashi Y, Penninger JM, Takahashi N
  • Organizer: 第26回内藤カンファレンス
  • Place of Presentation: 淡路島
  • Year and Date: 2009-11-05
• [Presentation] 細胞周期の停止した静止期破骨細胞前駆細胞(QOP)の性状解析-QOPはB細胞マーカーを発現している- (2009)
  • Author(s): 武藤昭紀, 溝口利英, 荒井敦, 小林泰浩, 中道裕子, 吉成伸夫, 宇田川信之, 高橋直之
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-24
• [Presentation] c-Fos遺伝子欠損マウスを用いた静止期破骨細胞前駆細胞(QOP)の解析-c-FosはRANKの発現を誘導しQOP分化を制御する- (2009)
  • Author(s): 荒井敦, 溝口利英, 武藤昭紀, 小林泰浩, 川原一郎, 中村美どり, 宇田川信之, 山田一尋, 高橋直之
  • Organizer: 第27回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-24