2010 Fiscal Year Final Research Report
Identifying antigens against antibodies produced in lesional synovium of rheumatoid arthritis and application of the enzyme-labeled antigen method
| Project/Area Number |
21890277
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
MIZUTANI Yasuyoshi Fujita Health University, 医学部, 助教 (10546229)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 酵素抗原法 / 抗体産生細胞 / 無細胞タンパク質合成系 / 関節リウマチ / 自己免疫疾患 / 自己抗原 / AlphaScreen / 免疫組織化学 |
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| Research Abstract |
To identify antigens against antibodies produced within rheumatoid arthritis (RA) synovitis, antigens recognized by antibodies in sera and synovial tissue extracts were screened with the AlphaScreen method. For screening, human protein library was constructed by the wheat germ cell-free protein synthesis system. As a result, a number of proteins were detected. A part of antigens showing high signals in synovitis tissue were applied to the enzyme-labeled antigen method. Specific antibodies against two antigens were visualized in plasma cells in RA synovitis. The present study indicated the usefulness of screening of the protein library with the AlphaScreen method for detecting antibodies in lesional tissue.
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Research Products
(6 results)
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[Journal Article] Specific in situ visualization of plasma cells producing antibodies against porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method.2010
Author(s)
Shinya Tsuge, Yasuyoshi Mizutani, Kazuhiro Matsuoka, Tatsuya Sawasaki, Yaeta Endo, Koji Naruishi, Hiroshi Maeda, Shogo Takashiba, Kazuya Shiogama, Ken-ichi Inada, Yutaka Tsutsumi
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Journal Title
J Histochem Cytochem. (In press)
Peer Reviewed
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