Identification of a new epitope of Cancer/Testis antigen KK-LC-1 and develop new adoptive immunotherapy

2010 Fiscal Year Final Research Report

• Project/Area Number: 21890298
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: SHIGEMATSU Yoshiki University of Occupational and Environmental Health, Japan, 医学部, 助教 (10546469)
• Project Period (FY): 2009 – 2010
• Keywords: 非小細胞肺癌 / 癌精巣抗原 / 免疫療法 / CTL

Research Abstract

Method of antibody titer against KK-LC-1 by ELISA using peptide of KK-LC-1 is established. Two patients showed high antibody titer against KK-LC-1 antigen. In these cases, we tried to identify a new epitope in the context of HLA-A2 or HLA-A24 restriction by the Reverse Immunology method, but it was not possible. Therefore, using the lymph node lymphocytes of an autologous patient, the CTL restricted HLA-A2 could be induced by a method of a peptide pulse to dendritic cells, and the new epitope could be identified.

Research Products

• [Journal Article] Clinical significance of cancer/testis antigens expression in patients with non-small cell lung cancer (2010)
  • Author(s): Shigematsu Y, Hanagiri T, Shiota H, Kuroda K, Baba T, Mizukami M, So T, Ichiki Y, Yasuda M, So T, Takenoyama M, Yasumoto K
  • Journal Title: Lung Cancer 68 Pages: 105-110
  • Peer Reviewed
• [Journal Article] Malignant mesothelioma-associated antigens recognized by tumor-infiltrating B cells and the clinical significance of the antibody titers (2009)
  • Author(s): Shigematsu Y, Hanagiri T, Kuroda K, Baba T, Mizukami M, Ichiki Y, Yasuda M, Takenoyama M, Sugio K, Yasumoto K
  • Journal Title: Cancer Sci 100 Pages: 1326-1334
  • Peer Reviewed
• [Presentation] Diagnostic markers for malignatn pleural mesothelinoma : Serum antibody against antigens recognized by antibodies produced from tumor infiltraring B cells (2010)
  • Author(s): Shigematsu Y, Kuroda K, Baba T, Ichiki Y, Ono K, Yasuda M, Uramoto H, So T, Takenoyama M, Hanagiri T, Yasumoto K
  • Organizer: The 10th International Conferenceoftne International Mesothelinoma Interest Group Kyoto
  • Place of Presentation: Japan
  • Year and Date: 20100831-20100903
• [Presentation] T細胞受容体移入γδT細胞による養子免疫療法への応用 (2010)
  • Author(s): 重松義紀,黒田耕志,馬場哲郎,永田好香,下川秀彦,小野憲司,浦本秀隆,竹之山光広,花桐武志,安元公正
  • Organizer: 第14回日本がん免疫学会総会
  • Place of Presentation: 熊本
  • Year and Date: 20100722-20100723
• [Presentation] T細胞受容体移入γδT細胞による治療効果の検討 (2010)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第31回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 20100520-20100521
• [Presentation] 局所進行期乳癌に対する術後補助療法としてのHER2蛋白ワクチンの検討 (2010)
  • Author(s): 黒田耕志,竹之山光広,馬場哲郎,重松義紀,宗哲哉,市来嘉伸,小野憲司,安田学,菅谷将一,浦本秀隆,宗知子,花桐武志,安元公正
  • Organizer: 第31回癌免疫外科研究会
  • Place of Presentation: 大阪
  • Year and Date: 20100520-20100521
• [Presentation] 悪性胸膜中皮腫関連抗原の同定と機能解析 (2010)
  • Author(s): 重松義紀,黒田耕志,馬場哲郎,宗哲哉,安田学,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20100513-20100514
• [Presentation] 肺癌に高発現する新規腫瘍抗原ribosomal protein L19の機能解析 (2010)
  • Author(s): 黒田耕志,竹之山光広,塩田広宣,馬場哲郎,重松義紀,宗哲哉,水上真紀子,市来嘉伸,小野憲司,安田学,菅谷将一,浦本秀隆,宗知子,花桐武志,安元公正
  • Organizer: 第27回日本呼吸器外科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 20100513-20100514
• [Presentation] 癌精巣抗原KK-LC-1を認識するT細胞受容体移入γδT細胞を用いた養子免疫療法への応用 (2010)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第110回日本外科学会定期学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20100408-20100410
• [Presentation] T細胞受容体移入γδT細胞を用いた特異的癌免疫療法の開発 (2009)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第63回日本呼吸器学会・日本結核病学会九州支部秋季学術講演会
  • Place of Presentation: 北九州
  • Year and Date: 20091126-20091127
• [Presentation] 石綿関連悪性腫瘍(胸膜中皮腫、肺癌)に対する新規バイオマーカー (2009)
  • Author(s): 安田学,花桐武志,重松義紀,黒田耕志,鬼塚貴光,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,安元公正
  • Organizer: 第63回日本呼吸器学会・日本結核病学会九州支部秋季学術講演会
  • Place of Presentation: 北九州
  • Year and Date: 20091126-20091127
• [Presentation] 腫瘍浸潤B細胞にて同定した悪性中皮腫関連抗原とその抗体価の臨床的意義 (2009)
  • Author(s): 重松義紀,黒田耕志,馬場哲郎,宗哲哉,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第63回日本呼吸器学会・日本結核病学会九州支部秋季学術講演会
  • Place of Presentation: 北九州
  • Year and Date: 20091126-20091127
• [Presentation] Diagnostic Markers for Malignant Pleural Mesothelioma : Serum Antibody against Antigens Recognized by Antibodies Produced from Tumor Infiltrating B cells (2009)
  • Author(s): Shigematsu Y, Yasumoto K
  • Organizer: 2009 4th China Medicai Biotech
  • Place of Presentation: Forum Dalian China
  • Year and Date: 20090808-20090810
• [Presentation] The identification of tumor antigens associated with asbestos-related lung cancer (2009)
  • Author(s): Hanagiri T, Yasuda M, Kuroda K, Shigematsu Y,Baba T, So T, Ono K, Uramoto H, So T, Takenoyama M, Yasumoto K
  • Organizer: 13th World Conferenceon Lung Cancer
  • Place of Presentation: San Francisco USA
  • Year and Date: 20090731-20090804
• [Presentation] T細胞受容体移入γδT細胞を用いた特異的癌免疫療法の開発 (2009)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,市来嘉伸,小野憲司,浦本秀隆,宗知子,竹之山光広,花桐武志,安元公正
  • Organizer: 第13回日本がん免疫学会総会
  • Place of Presentation: 北九州
  • Year and Date: 20090624-20090625
• [Presentation] T細胞受容体移入γδT細胞を用いた特異的癌免疫療法の開発 (2009)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,宗哲哉,市来嘉伸,安田学,浦本秀隆,宗知子,竹之山光広,花桐武志,杉尾賢二,安元公正
  • Organizer: 第30回癌免疫外科研究会
  • Place of Presentation: 久留米
  • Year and Date: 20090521-20090522
• [Presentation] 悪性胸膜中皮腫における腫瘍抗原の同定と新規腫瘍マーカーとしての意義 (2009)
  • Author(s): 重松義紀,塩田広宣,黒田耕志,馬場哲郎,永田好香,水上真紀子,市来嘉伸,安田学,竹之山光広,花桐武志,杉尾賢二,安元公正
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20090402-20090404
• [Presentation] 腫瘍抗原特異的T細胞受容体(TCR)遺伝子移入による抗腫瘍エフェクター細胞の誘導(第2報) (2009)
  • Author(s): 竹之山光広,重松義紀,塩田広宣,黒田耕志,馬場哲郎,市来嘉伸,花桐武志,安元公正
  • Organizer: 第62回日本呼吸器学会・日本結核病学会九州支部春季学術講演会
  • Place of Presentation: 那覇
  • Year and Date: 2009-05-23