Development of a new molecular typing method to characterize Salmonella enterica serotype Montevideo, Infantis, and Schwarzengrund isolated in Osaka, Japan.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21890317
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Public health/Health science
• Research Institution: Osaka Prefectural Institute of Public Health
• Principal Investigator: HARADA Tetsuya Osaka Prefectural Institute of Public Health, 感染症部, 研究員 (70516723)
• Project Period (FY): 2009 – 2010
• Keywords: 公衆衛生 / 分子疫学解析法 / サルモネラ

Research Abstract

We aimed to develop a new molecular analysis method for differentiating Salmonella enterica serovar Montevideo, Salmonella Infantis and Salmonella Schwarzengrund isolates in Osaka Prefecture, Japan. We described a multiple-locus variable-number tandem repeats (VNTR) analysis (MLVA) method based on agarose gel electrophoresis separation of polymerase chain reaction products of three simple VNTR loci in Salmonella Montevideo. We believe that the MLVA method can facilitate assessment of the molecular epidemiology of Salmonella Montevideo isolates in light of its present utility in laboratories without a PFGE apparatus or capillary array electrophoresis devices.

Research Products

• [Journal Article] Molecular Epidemiological Investigation of a Diffuse Outbreak Caused by Salmonella enterica Serotype Montevideo Isolates in Osaka Prefecture, Japan.
  • Author(s): Harada, T., Sakata, J., Kanki, M., Seto, K., Taguchi, M., Kumeda, Y.
  • Journal Title: Foodborne Pathog.Dis. in press
  • Peer Reviewed