2010 Fiscal Year Final Research Report

Development of a new molecular typing method to characterize Salmonella enterica serotype Montevideo, Infantis, and Schwarzengrund isolated in Osaka, Japan.

Research Project

Project/Area Number	21890317
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Public health/Health science
Research Institution	Osaka Prefectural Institute of Public Health
Principal Investigator	HARADA Tetsuya Osaka Prefectural Institute of Public Health, 感染症部, 研究員 (70516723)
Project Period (FY)	2009 – 2010
Keywords	公衆衛生 / 分子疫学解析法 / サルモネラ
Research Abstract	We aimed to develop a new molecular analysis method for differentiating Salmonella enterica serovar Montevideo, Salmonella Infantis and Salmonella Schwarzengrund isolates in Osaka Prefecture, Japan. We described a multiple-locus variable-number tandem repeats (VNTR) analysis (MLVA) method based on agarose gel electrophoresis separation of polymerase chain reaction products of three simple VNTR loci in Salmonella Montevideo. We believe that the MLVA method can facilitate assessment of the molecular epidemiology of Salmonella Montevideo isolates in light of its present utility in laboratories without a PFGE apparatus or capillary array electrophoresis devices.