SCN2A自閉症に関する膜電位イメージングによる細胞体樹状突起シグナリングの解析

2021 Fiscal Year Annual Research Report

• Project/Area Number: 21F20712
• Research Institution: Okinawa Institute of Science and Technology Graduate University
• Principal Investigator: Kuhn Bernd 沖縄科学技術大学院大学, 光学ニューロイメージングユニット, 教授 (90599557)
• Co-Investigator(Kenkyū-buntansha): LI MELODY 沖縄科学技術大学院大学, 光学ニューロイメージングユニット, 外国人特別研究員
• Project Period (FY): 2021-09-28 – 2023-03-31
• Keywords: Scn2a/Nav1.2 / neurological disorders / In vivo calcium imaging

Outline of Annual Research Achievements

1. Establish a novel mouse model that will be a valuable tool to study pathomechanisms underlying Scn2a neurological disorders
2. Study has been accepted for poster presentation at Neuro2022 conference

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

A novel Scn2a mouse model was created using antisense oligonucleotide technology. A battery of behavioural studies were performed and found the ASO treated mice mirror clinical features observed in patients with Scn2a neurological disorders. Simultaneous calcium imaging and electrocorticography (ECoG) was completed in a cohort of animals. Data analysis in progress.
Preliminary results suggest ASO treated mice have lowered power spectrum density power, specifically in the lower frequencies, when challenged with a pro-convulsant.

Strategy for Future Research Activity

The plan is to complete calcium imaging analysis then segment both imaging and ECoG signal by running, resting and post-proconvulsant state. The data would reveal potential differences between ASO treated and control mice at a neural network level. This is the first study to explore in vivo neural network activities in a Scn2a mouse model and we expect outcomes to result in a publication.