2021 Fiscal Year Annual Research Report
What are the geometrical factors that regulate the propagation of calcium signals in astrocytes?
| Project/Area Number |
21F21733
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
DeSchutter Erik 沖縄科学技術大学院大学, 計算脳科学ユニット, 教授 (00466319)
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| Co-Investigator(Kenkyū-buntansha) |
DENIZOT AUDREY 沖縄科学技術大学院大学, 計算脳科学ユニット, 外国人特別研究員
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| Project Period (FY) |
2021-07-28 – 2023-03-31
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| Keywords | neuroscience / glia / astrocytes / calcium signaling / modeling / reaction diffusion / 3D reconstruction / interdisciplinary |
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| Outline of Annual Research Achievements |
My work has resulted in the publication of 2 conference proceedings articles (DOI: 10.1109/ISBI48211.2021.9433984 and 10.1145/3477206.3477456) and 2 preprints (DOI:10.1101/2022.02.28.482292 and 10.20944/preprints202201.0460.v1). I presented at 7 international events: 3 invited talks (99th Annual Meeting of the Physiological Society of Japan, Sendai, March 2022 ; 8th ACM International Conference on Nanoscale Computing and
CommunicationVirtual Conference, Sept 2021 and annual Computational Neuroscience meeting, July 2021), 2 talks (The 44th Annual Meeting of the Japan Neuroscience Society, Kobe, July 2021 and the 1st Virtual Conference of the European Society for Neurochemistry, May 2021) and 2 posters (XV European Meeting on Glial Cells in Health and Disease, GLIA 2021, online, July 2021 ; 1st ESN Virtual Conference, May 2021) where I won a best poster award). I organized 2 international workshops: annual Computational Neuroscience meeting, July 2021, "Computational approaches for studying astrocyte dynamics and astrocyte-neuron communication". and 1st Virtual Conference of the European Society for Neurochemistry, May 2021, "Let’s join forces - Bridging the gap between experimental, computational and data sciences to disentangle astrocyte calcium activity".
These results improve understanding of calcium dynamics in astrocytes, it has been reported that many neurological diseases shown abnormal astrocyte calcium dynamics. Moreover, the last workshop promoted interaction between experimentalists and modelers in the field, which is essential for future progress.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
My research in FY2021 focused on studying the effect of geometrical features on the propagation of calcium signals in astrocytic branchlets (1st aim of the research project). Spatial stochastic simulations were performed in idealized branchlet geometries constructed based on super-resolution microscopy data (Denizot et al., 2021, bioRxiv), in idealized cylindrical geometries to test the effect of the distribution of calcium sources on local activity (published in ACM NanoCom 2021 proceedings, DOI: 10.1145/3477206.3477456) and in realistic single branchlet geometries extracted from electron microscopy (Denizot et al., bioRxiv, 2022). Those studies contribute to a better understanding of the spatial factors that regulate signal propagation in single astrocyte branchlets.
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| Strategy for Future Research Activity |
My plan for FY2022 is to create three dimensional geometries of networks of astrocytic branchlets to investigate the effect of the architecture of the so-called ‘spongiform domain’ of astrocytes on signal propagation in the cell. I obtained astrocyte 3D reconstructions from Dr. C. Cali, University of Turin, Italy. I aim at developing innovative algorithms to alter the geometrical properties of astrocyte branchlet networks with Blender software, similarly to the computational scripts developed during FY2021 to manipulate the geometrical features of calcium sources within astrocytic branchlets (Denizot et al., bioRxiv, 2022, DOI: 10.1101/2022.02.28.482292). I will then run simulations in those geometries to investigate calcium signal propagation in the spongiform domain of astrocytes.
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[Journal Article] Simulation of Astrocytic Calcium Dynamics in Lattice Light Sheet Microscopy Images2021
Author(s)
Badoual, A., Arizono, M., Denizot, A., Ducros, M., Berry, H., Nagerl, U.V. and Kervrann, C.
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Journal Title
2021 IEEE 18th International Symposium on Biomedical Imaging (ISBI)
Volume: -
Pages: 135-139
DOI
Peer Reviewed / Int'l Joint Research
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