Development of primitive heart model using iPS cell derived cardiomyocytes and construction of cardiac mathematical model

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H01215
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 18010:Mechanics of materials and materials-related
• Research Institution: Kyushu University
• Principal Investigator: Todo Mitsugu 九州大学, 応用力学研究所, 准教授 (80274538)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: iPS細胞 / 心筋細胞 / 細胞シート / 3Dバイオプリンター / 粘弾性

Outline of Final Research Achievements

Cardiac cell sheets were successfully developed using iPS cells derived cardiomyocytes for the development of a fundamental heart model. It was found that electrical stimulation can be used to control the pulsation behavior. Such kind of stimulation was also found to change the viscoelastic hysteresis loops of the stress-strain response. The flow behavior in the inside of a hybrid construct of PDMS tube and iPS cardiac sheet was also examined. It was found that the flow velocity change can be modeled using a theoretical model developed by considering the elastic deformation of tube with pulsation. 3D bioprinter was also used to construct the scaffolds for the fundamental heart model. This kind of scaffold will be able to be used to construct simple heart-like models with use of iPS cardiac sheets.

Free Research Field

生体医工学

Academic Significance and Societal Importance of the Research Achievements

iPS細胞由来心筋細胞シートを用いた心臓疾患の治療は、すでに臨床研究の段階であるが、その力学的特性に関する研究はほとんど行われていない。本研究は、電気刺激により拍動挙動の制御が可能であることを見出し、さらに電気刺激が粘弾性的応力-ひずみ応答特性に影響を与えることを明らかにした。これらの力学特性に関する知見は、心筋シートの臨床応用において有用となることが期待される。さらに、iPS心筋シートとPDMSチューブを組み合わせた３D構造体の流動特性、ならびに3Dバイオプリンターで作製した３D足場構造体に関する知見は、今後の心臓原始形態モデル開発に向けて重要な知見となると考えられる。