2023 Fiscal Year Final Research Report
Development of microfluidic system for transplantation of characteristics of hardly culturable bacteria
| Project/Area Number |
21H01284
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 20020:Robotics and intelligent system-related
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金子 真也 東京工業大学, 生命理工学院, 助教 (10399694)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 細胞融合 / マイクロギャップ / 細菌数制御 / 一括細胞淘汰 / 繰り返し |
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| Outline of Final Research Achievements |
We developed microfluidic devices for cell fusion of bacteria with low damages and for expressing or strengthening characteristics of fused bacteria. As for the cell fusion device, we developed microscaled gap opposing electrodes to reduce the voltage to induce cell fusion, and a single bacterium-sized narrow gap to transport a single bacterium to reduce the randomness of gene expression. As for expressing and strengthening characteristics, we developed techniques to control and apply different stresses of temperature by serially connected heater and concentration of antibacterial drug by gradient generator. For the cyclic operation of expressing and strengthening fused bacterial characteristics, we also achieved equal division of a droplet by a geometrical design, and sequential microfluidic transfer of equally divided droplets.
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| Free Research Field |
マイクロ流体
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| Academic Significance and Societal Importance of the Research Achievements |
低ダメージ細胞融合技術は細胞に大きな分子やゲノムを導入できる技術であり、細菌の遺伝子改変技術を飛躍させる可能性がある。その際、これまでの細胞融合は大量の細胞同士で行っており、得られる形質がランダムであったが、異種細胞の細胞数を制御することで、そのランダム性を大幅に低減できる。融合細胞の特性発現と強化の技術は、刺激に合わせて形質発現する可能性を高めるため、ランダムな形質発現なために諦められていた形質を融合細胞に付与できる可能性が出てくる。バイオテックにおいて遺伝子操作は不可欠であり、本研究の成果はそれに対して大きな影響を与える可能性が高い。
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