Development of Super Sensitive Detection Systems for Biomolecules Utilizing DNA Origami Molecular Machines

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H01774
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28040:Nanobioscience-related
• Research Institution: Kansai University
• Principal Investigator: Kuzuya Akinori 関西大学, 化学生命工学部, 教授 (00456154)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: DNAオリガミ / 人工抗体 / 分子機械 / 超解像イメージング法

Outline of Final Research Achievements

The initial goal of this project was to develop artificial antibodies that use "DNA Origami molecular machine," which functions through "mechanical motion," as the main body, and to develop an ultra-sensitive detection method to detect biomolecules at the single molecule level by real-time observation of the structural changes of this molecular machine using a super-resolution imaging technique. As a result, we have established a method to introduce antibody fragments into DNA Origami structures as substrate recognition sites, and have implemented a bioluminescence resonance energy transfer system into DNA Origami molecular machines as a signal transmitting site to visualize structural changes of DNA Origami molecular machines that can also be applied to super-resolution imaging technique.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

抗体は巨大、かつ複雑なタンパク質であり、その製造には哺乳動物細胞の培養が必要となるなど、抗体医薬などに応用するためには非常にコストが高いことが従来から問題視されてきました。この問題を解決するために本研究では、比較的安価に生産できる抗体断片をDNAの足場上で組み上げることで人工抗体を構築するための道筋をたてました。また、この人工抗体を超高感度で検出するための光技術もあわせて開発に成功しました。