Analysis of crosstalk between stress response pathways by multicolor real-time bioluminescence measurement

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02083
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37030:Chemical biology-related
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Nakajima Yoshihiro 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (10291080)
• Co-Investigator(Kenkyū-buntansha): 横田 一道 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (50633179) ; 宮田 椋 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (20981426) ; 于 躍 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (90881631)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ルシフェラーゼ / ストレス応答 / クロストーク / 細胞内ネットワーク / リアルタイム発光測定

Outline of Final Research Achievements

The objective of this study is to capture the dynamic changes in stress response pathways during stress induction using our originally developed multicolor real-time bioluminescence measurement method, and to elucidate the crosstalk between stress response pathways. Furthermore, we aim to obtain data that will contribute to the analysis of the network structure. In this study, we established a luminescent cell line to analyze typical intracellular stress responses such as oxidative stress response and inflammation response. Furthermore, we have established a system to measure these dynamic changes in real-time. We have clarified the effects of phytochemicals, drugs, and physicochemical stresses on each stress response pathway. Furthermore, we analyzed the crosstalk between stress responses involved in the adaptive response to stress.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、細胞の恒常性を維持し、細胞内外からのストレスに対して防御するための手段である適応応答機構の学術的理解を深化させるとともに、構築した解析システムの社会普及によりライフサイエンス研究分野およびバイオテクノロジー関連産業における生理活性物質の開発の加速に貢献することが期待される。