Elucidation and development of biosynthesis and action mechanism of various novel bacteriocins of lactic acid bacteria

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02107
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Kyushu University
• Principal Investigator: Zendo Takeshi 九州大学, 農学研究院, 准教授 (50380556)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: バクテリオシン / 抗菌ペプチド / 乳酸菌 / 生合成機構 / 抗菌タンパク質

Outline of Final Research Achievements

From lactic acid bacteria isolated from diverse sources, many novel bacteriocins characteristic in structure and activity, especially various class II bacteriocins and class III bacteriocins (antimicrobial proteins), were identified. Biosynthetic gene clusters for novel bacteriocins including circular and glycosylated bacteriocins were determined and characterized for self-immunity, secretion, and maturation. A multiple bacteriocin transporter were demonstrated for its availability in secretion of various antimicrobial peptides.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

安全な抗菌物質としての様々な用途での利用が期待される乳酸菌バクテリオシンについて、新しい生産株や新しい構造を有するものを多数見出した。また、種々の新奇乳酸菌バクテリオシンが有する特徴的な生合成機構や作用機構の一端を明らかにした。さらには、乳酸菌バクテリオシンが有する生合成機構を利用した、抗菌ペプチドの分泌発現、評価系の基盤を構築した。これらの成果により、用途に応じた乳酸菌バクテリオシンの選択が可能となり、より効果的な抗菌ペプチドの創出への展開が期待される。