2023 Fiscal Year Final Research Report
Understanding the core structures and dynamics of membrane protein complexes related in productions of valuable compounds from plants
| Project/Area Number |
21H02115
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38030:Applied biochemistry-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
戸澤 譲 埼玉大学, 理工学研究科, 教授 (90363267)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 天然ゴム / フラボノイド / メタボロン / イソプレノイド / 植物特化代謝 / 二次代謝産物 / 結晶構造 |
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| Outline of Final Research Achievements |
Plant secondary metabolites (so called plant specialized metabolites) have been thought to be efficiently synthesized by a membrane-bound enzyme complex which is termed as metabolon. However, complex structures, stoichiometry and interface of the complex have not yet been clarified because of weak interactions between metabolon components on membrane environment. So far, no metabolon have been isolated and crystallized as a complex. In this project, flavonoid and isoprenoid (especially natural rubber) biosynthetic enzymes, that are known to form metabolon, were investigated. As a result, we successfully determined the stoichiometry and conditions that are required for the enzymatic activity of the core complex for natural rubber biosynthesis. Moreover, the core complex for flavonoid biosynthetic pathway were crystallized as a complex for the first time. This complex structure revealed the interface which was supported by crosslinking experiments.
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| Free Research Field |
構造生物学
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| Academic Significance and Societal Importance of the Research Achievements |
植物が生産する二次代謝産物は、近年は植物特化代謝産物とよばれ、薬用化合物の宝庫である。また、植物特化代謝産物の一種である天然ゴムは、化学成分としては普遍的なイソプレンという繰り返しから合成されるものでありながら、ポリマーとしてのゴムは、特定のゴム生産植物しか合成できない。そのため、植物は奇跡の化学工場とも言われているが、そのキーとなるのが酵素複合体メタボロンの形成である。これらの作用機序、複合体構造、量論比、相互作用界面に関する知見を得ることは、基礎科学として新規性が高いが、微生物などの他の宿主で植物特化代謝の代替生産系を構築する応用上、必要不可欠な情報であり、大きな波及効果がある。
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