Development of genetic analysis methods for hexaploid chrysanthemum

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02189
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39030:Horticultural science-related
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: Shirasawa Kenta 公益財団法人かずさDNA研究所, 先端研究開発部, 室長 (60527026)
• Co-Investigator(Kenkyū-buntansha): 住友 克彦 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門, 上級研究員 (70391406)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: キク / 高次倍数体 / 遺伝解析 / ゲノム / トランスクリプトーム / エピゲノム

Outline of Final Research Achievements

Many of the phenotypes of higher polyploids may be controlled not only by genotype but also by allele-specific transcription. Using chrysanthemum, a hexaploid plant, as a model, this study aimed to elucidate the mechanisms that cause allele-specific transcription in the polyploid genomes. We cultivated a mapping population of chrysanthemum to collect phenotype data. Simultaneously, transcriptome and epigenome data were also obtained. Using the genome sequence of Chrysanthemum seticuspe, a closely related diploid wild species, as a reference sequence, we mapped the data and quantified the transcription levels of genes while revealing the distortion of allele frequencies in transcripts. Additionally, we constructed the reference genome sequence of hexaploid chrysanthemum.

Free Research Field

園芸科学関連

Academic Significance and Societal Importance of the Research Achievements

高次倍数体の表現型を高精度に推定するためには、従来の二倍体を対象とした分子遺伝学研究やゲノム研究の手法が適用できない場合が多い。本研究成果により高次倍数体の実用的な遺伝解析研究が大きく進展し、キクに限らずサツマイモやカキ、キウイフルーツ、イチゴなどの遺伝という現象をより深く理解できるようになることが期待される。