2023 Fiscal Year Final Research Report

Search for virulence factors of Bursaphelenchus xylophilus using a genome-wide association study

Research Project

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Project/Area Number	21H02249
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 40010:Forest science-related
Research Institution	Forest Research and Management Organization
Principal Investigator	Akiba Mitsuteru 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員等 (50353553)
Co-Investigator(Kenkyū-buntansha)	菊地泰生東京大学, 大学院新領域創成科学研究科, 教授 (20353659) 新屋良治明治大学, 農学部, 専任准教授 (30802798)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	マツノザイセンチュウ / 病原力 / GWAS / マツ材線虫病
Outline of Final Research Achievements	We obtained the information necessary to conduct a genome-wide association study (GWAS) to search for genetic factors associated with phenotypic traits, such as virulence, of the pinewood nematode, causal agent of pine wilt disease. As a result, it was inferred that no population structure is recognized in the pinewood nematode in Japan. In addition, more than 800,000 SNPs and phenotypic trait information (virulence, proliferation, and sex ratio) were obtained for each nematode isolates. All isolates studied were cryopreserved for future research needs.
Free Research Field	森林病理学
Academic Significance and Societal Importance of the Research Achievements	マツ材線虫病に関しても今後GWASを用いた研究が進むようになると想定されるが，本課題はそのパイロット的な研究である。日本国内の広範囲から分離された株について高精度のSNP情報を取得できたが、あらゆる表現型形質に対するGWASにこれらの株を使用することが可能であり，学術的意義も高いと考えている。