Comprehensive and precise classification and characterization of Coding/Non-coding RNA

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02404
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: Nagoya University (2023); Kyushu University (2021-2022)
• Principal Investigator: Matsumoto Akinobu 名古屋大学, 理学研究科, 教授 (60741519)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: Long non-coding RNA / Coding RNA / 翻訳プロファイリング

Outline of Final Research Achievements

Having shown that a fraction of long non-coding RNAs (lncRNAs) translate polypeptides, we next set out to identify the true lncRNAs. Using various translation profiling methods, we were able to accurately classify coding/non-coding RNAs and identified a group of RNAs without 5'caps. Although these RNAs do not have a 5'cap structure, they were unexpectedly observed to interact with the ribosome, suggesting that they may be translated. We have constructed a reporter system to artificially express these RNAs and will be able to study the properties of these RNAs in detail.

Free Research Field

Long non-coding RNA

Academic Significance and Societal Importance of the Research Achievements

新種のRNAを同定したことはRNAの研究領域において非常に意義が高い。またそれら新種のRNAを解析するための実験系を構築したことから、今後これらRNAの意義がさらに明らかになることが期待される。