2023 Fiscal Year Final Research Report
Development of optical large-scale membrane potential measurement methods based on communication technology
| Project/Area Number |
21H02437
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 多光子顕微鏡 / 神経活動計測 / 膜電位イメージング |
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| Outline of Final Research Achievements |
Various investigations were carried out to develop a method for large-scale measurement of neural activity using membrane potential-responsive fluorescent proteins. First, a home-built multiphoton microscope was designed and constructed to study the measurement conditions, enabling fluorescent images to be acquired from neurons. Next, assuming that the target neurons move during the measurement, we developed an algorithm for estimating and tracking the cell positions and verified the principle experimentally. Furthermore, the implementation form of the multi-point detection method using available optical elements such as objective lenses and scanners was investigated, and the measurement frequency in a specific implementation form was estimated by numerical simulation and theoretical calculation, and conditions were found under which approximately 2000 mouse neurons could be measured at approximately 1 kHz.
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| Free Research Field |
バイオフォトニクス
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は脳内のニューラルネットワークのふるまいを実際に記録するための手段を検討するものであり、脳に対する基本的な理解および神経疾患の機序解明につながるという点において理学、あるいは医科学としての学術的意義および社会的意義を有する。さらに、本研究では従来標準的に用いられてきたイメージングに基づく神経活動計測手法の限界を打破するための新たな計測手段を追究するものであり、工学の観点からも新たな計測学を開拓するという点で学術的意義を有する。
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