2021 Fiscal Year Annual Research Report
Regulation of hemangioblst development by partial Epithelial Mesenchymal Transition
| Project/Area Number |
21H02490
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| Research Institution | Kumamoto University |
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Principal Investigator |
Sheng Guojun 熊本大学, 国際先端医学研究機構, 特別招聘教授 (20399439)
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| Co-Investigator(Kenkyū-buntansha) |
永井 宏樹 熊本大学, 国際先端医学研究機構, リサーチ・スペシャリスト (80772508)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | EMT / chicken / hemangioblast / hematopoiesis / vasculogenesis |
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| Outline of Annual Research Achievements |
In FY2021, we have investigated the expression of two key EMT regulators
Snai2 and Zeb2 in hemangioblast development. We have also mad expression constructs of these two genes (Snai2 and Zeb2) for functional analysis. In addition, we have analyzed the promoter regions of these two genes and identified the transcription start sites. With these data, we also cloned constructs for CRISPR-Cas9 mediated gene activation and inhibition for Snai2 and Zeb2.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The work is making progress as planned.
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| Strategy for Future Research Activity |
In FY2022, we will analyze in detail the role of these two EMT regulators
(Snai2 and Zeb2) in regulating both hemangioblast fate (as assayed by several blood and endothelial markers, as we have used previously) and in regulating cell polarity markers of precursors (before blood and vessel fate
segregation) and during early lineage segregation (i.e., for the blood
lineage: when blood marker is being turned on, but before blood cells loose cell-cell contact with each other and with endothelial cells; for the endothelial lineage: when endothelial specific cell-cell junctional interactions are being established). By the end of FY2022, we expect to establish the timeline and hierarchy of EMT transcriptional regulation, lineage transcriptional regulation, and morphological features of blood and endothelial cells during the critical phase of their lineage specification and segregation.
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