• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2023 Fiscal Year Final Research Report

Elucidation of Molecular Sorting Mechanisms using Cryo-electron microscopy

Research Project

  • PDF
Project/Area Number 21H02654
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 48010:Anatomy-related
Research InstitutionUniversity of Yamanashi

Principal Investigator

Oda Toshiyuki  山梨大学, 大学院総合研究部, 教授 (20569090)

Co-Investigator(Kenkyū-buntansha) 柳澤 春明  東京大学, 大学院医学系研究科(医学部), 講師 (70466803)
新森 英之  山梨大学, 大学院総合研究部, 准教授 (40311740)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywordsクライオ電子顕微鏡 / 繊毛
Outline of Final Research Achievements

Cilia are essential cellular organelles necessary for cell signaling and motility. The central structure of cilia, the axoneme, adopts the so-called '9+2' structure composed of nine peripheral microtubules and two central pair microtubules. However, the molecular mechanisms that construct this complex structure remain largely unknown. This study identifies a molecule at the base of one of the main projections of the central pair microtubules, the C2a structure, and demonstrates that its presence is essential for the construction of the C2a structure. Additionally, by utilizing the incorporation of the antibiotic puromycin into nascent proteins, we have revealed the existence of a precursor pool at the base of cilia. Furthermore, by genetically manipulating the carboxyl-terminal ends of tubulin, we have shed light on some aspects of the construction mechanism of the central pair microtubules.

Free Research Field

構造生物化学

Academic Significance and Societal Importance of the Research Achievements

本研究は繊毛を持つ緑藻クラミドモナスを用いて、繊毛の動きと構造に欠かせないタンパク質の働きを明らかにしました。繊毛は呼吸器や生殖系など人体の多くの部位で重要な役割を果たしており、この研究は病気の治療法の開発に貢献する可能性を秘めています。具体的には、抗生物質の特殊な性質を利用した新規分析法を用いて繊毛タンパク質の合成を追跡し、また遺伝子操作により繊毛の微細構造を解明する手法を開発しました。これらの成果は、繊毛異常が引き起こす病気の理解を深め、治療に向けた新たな道を開く基盤となります。

URL: 

Published: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi