2023 Fiscal Year Final Research Report
Regulation of signal transduction by TRIM family ubiquitin ligases
Project/Area Number |
21H02674
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 48040:Medical biochemistry-related
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Research Institution | Hokkaido University |
Principal Investigator |
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Keywords | ユビキチン / TRIMタンパク質 / シグナル伝達 |
Outline of Final Research Achievements |
Recently, the importance of TRIM family ubiquitin ligases in intracellular signal transduction related to immune responses and cancerization has been clarified. In particular, TRIM family ubiquitin ligases have been shown to be involved not only in cell proliferation but also in signal transduction systems that control cell differentiation processes and autophagy regulation, and have recently attracted attention in many fields of intracellular signal transduction systems. In this application, we comprehensively analyzed the intracellular network system centered on TRIM family ubiquitin ligases using comprehensive knockdown screening and proteomics methods specific to ubiquitination.
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Free Research Field |
生化学
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Academic Significance and Societal Importance of the Research Achievements |
これまでは各TRIMタンパク質の機能が個別に報告されてきたが、本申請研究では全TRIMタンパク質のsiRNAやプロテオミクス的解析等を使用することで、さまざまな細胞内シグナルを網羅的に解析し、TRIMファミリー全体のシステム構造の解明を進めた。今後、TRIMファミリーによる翻訳後修飾(特にユビキチン化)の生理機能および疾患との関連の解明という点で、感染症、自己免疫疾患、がん化、細胞分化、個体発生、神経変性疾患など、医学における総合的理解に貢献する可能性が高い。
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