2023 Fiscal Year Final Research Report
Spatial transcriptome analysis using photoreleasing barcodes
| Project/Area Number |
21H02790
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 50020:Tumor diagnostics and therapeutics-related
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高村 禅 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (20290877)
藤渕 航 京都大学, iPS細胞研究所, 教授 (60273512)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | single cell / spatial transcriptome / microenvironment / cancer / Genome mutation |
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| Outline of Final Research Achievements |
The aim of this study is to create barcode beads using photoresponsive artificial nucleic acids and capture beads strung in the same space to enable the observation of tissue location information and the measurement of multiple elements such as gene expression and genome mutations in one cell. So far, parts of arbitrary DNA, such as Kras and several mutated sites of Tp53 in the genome at the one-cell level, have been successfully observed. On the other hand, with regard to the studies that have been carried out to maintain and analyse the spatial information of cells in tissues, we have also succeeded in analysing mouse brain tissue and breast cancer tissue with the information of the cell clusters. Currently, we have partially succeeded in observing the positional information of nucleic acid-trapped beads, but further validation is required.
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| Free Research Field |
分子生物学、免疫学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の目的は、光応答性人工核酸を使ったバーコードビーズを開発・作製し、同一空間で紐付けされたキャプチャービーズを使用することにより、1細胞の遺伝子発現や変異などの複数の要素を測定可能とすることである。本法は、組織での位置情報を持った1細胞情報の取得と細胞間の相互作用の関係を明らかでき、この研究はまさに革新的でありマーカー遺伝子の探索、創薬ターゲット、診断、治療法にとって非常に重要である。さらに、我々の開発する方法は、研究室，臨床サイドでも安価かつ簡単に使用できることを利点とする。
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