2023 Fiscal Year Final Research Report
Development of technologies for pathway-specific manipulation and application to disease models in primates
| Project/Area Number |
21H02798
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 51010:Basic brain sciences-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 脊髄損傷 / サル / 機能回復 / ウイルスベクター / DREADD |
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| Outline of Final Research Achievements |
In this study, we developed a technique that can reversibly manipulate a wide range of neural circuits by combining CED for widespread virus vector injection, double viral vector technique for selecting specific pathways, and DREADDs for reversible pathway manipulation. Furthermore, we applied this circuit manipulation technique to a spinal cord injury model in a non-human primate. During the recovery process from spinal cord injury, blocking the interhemispheric pathway in the early stages of recovery resulted in a significant decrease in the success rate of the reach and grasp task. These results directly demonstrated the contribution of the interhemispheric pathway to functional recovery.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
選択的回路操作の実験は、齧歯類を対象としたモデル動物で盛んに行われているが、霊長類を対象としたウイルスベクター注入実験の成功例は少ない。本研究課題で開発された技術は広汎な神経回路の操作を可能にするものであり、操作中の脳活動も記録することが可能である。このような技術は行動に影響を与える新規のネットワークの抽出に貢献でき、さらに病態モデルに応用することで、リハビリテーションの効果の評価や促進、新たな治療の創出への応用につながることも期待される。
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