2023 Fiscal Year Final Research Report

Radiation-induced oxidative modification response of serum albumin and its application to dose/damage prediction

Research Project

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Project/Area Number	21H02860
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 52040:Radiological sciences-related
Research Institution	Hirosaki University
Principal Investigator	Kashiwakura Ikuo 弘前大学, 保健学研究科, 特任教授 (00177370)
Co-Investigator(Kenkyū-buntansha)	多田羅洋太弘前大学, 医学研究科, 助教 (00443995) 山口平弘前大学, 保健学研究科, 助教 (00782822) 伊藤勝博弘前大学, 災害・被ばく医療教育センター, 教授 (20322951) 辻口貴清弘前大学, 災害・被ばく医療教育センター, 助教 (90737454)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	電離放射線 / 生物学的線量評価 / 血清アルブミン / 酸化修飾応答
Outline of Final Research Achievements	LC/MS/MS analysis of blood from residents of high natural radiation areas (average 70 mSv/year) in Indonesia, mainly due to chronic inhalation exposure to radon and tron, identified several dose-dependent oxidative modification peptides, apart from the 34th cysteine (Cys-34) oxidation of serum albumin. This oxidative modification response of SA was also observed in mice after single irradiation (0.5 to 3 Gy), even in acute external exposure. Subsequently, when the oxidative modification response of SA was analysed before and after CT examinations of multiple trauma patients transported to the Hirosaki University Advanced Emergency Centre, a specific response was observed even at low doses averaging approximately equal to 30 mSv. Based on these results, the oxidative modification response of SA is expected to be utilised as a biodosimetry marker for acute low-dose exposure.
Free Research Field	放射線生物学
Academic Significance and Societal Importance of the Research Achievements	血清中に最も高濃度に存在する血清アルブミン（SA）は，活性酸素種により酸化や糖化などの修飾を受け，特にSAの34番目のシステイン（Cys-34）が酸化される．我々は、SAのCys-34酸化とは別の線量依存的な酸化修飾応答を見出したが、放射線は強い酸化ストレスであり，他の酸化ストレス関連疾患（感染症、ガン、糖尿病や老化等）においても特異的な修飾応答が生じている可能性が強く示唆される．今後は、ヒトに特異的なSAの酸化修飾応答ペプチドの迅速かつ高精度な検出方法（デバイス等）開発や様々な疾患との関連性の検討が求められる。