Generation of purely pluripotent stem cell derived lung organ

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02923
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: Niigata University
• Principal Investigator: SAIJO YASUO 新潟大学, 医歯学系, 教授 (10270828)
• Co-Investigator(Kenkyū-buntansha): 阿部 学 新潟大学, 脳研究所, 准教授 (10334674) ; 周 ケイリョウ 新潟大学, 医歯学系, 准教授 (10770232) ; 笹岡 俊邦 新潟大学, 脳研究所, 教授 (50222005) ; 小田 佳奈子 新潟大学, 脳研究所, 助教 (60708212) ; 味岡 洋一 新潟大学, 医歯学系, 教授 (80222610)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 肺再生 / ES細胞 / ガストロイド / 胚盤胞補完法

Outline of Final Research Achievements

This study aims to ultimately create lungs derived 100% from embryonic stem cells. Mouse ES cells were cultured in three dimensions to attempt the creation of pseudo-embryos. ES cells cultured in ESLIF medium were started in a 96 well plate, and the culture was continued with a change to N2B27 medium containing Chiron, promoting the development and differentiation of gastruloids. As a result of trying various conditions, the ES cells aggregated and formed an individual entity. Histologically, the development of the primitive gut or lung buds was not confirmed. Also, genetic analysis did not confirm the expression of genes involved in lung development. In addition, research on lung regeneration by blastocyst complementation method was continued. By transplanting rat ES cells into mice with lung deficiencies, lung regeneration was successful in five cases. Further analysis confirmed the creation of lungs derived from rat ES cells

Free Research Field

再生医学

Academic Significance and Societal Importance of the Research Achievements

移植に適した肺は数が少なく、多くの待機患者さんがいます。この移植可能な肺臓器を作ることを最終目標としています。胚性幹細胞から肺臓器を作成する方法として、①肺が欠損した動物内で作る胚盤胞補完法と、②胚性幹細胞から疑似胚を作成して肺を作る方法について研究を継続してきています。①では小動物ですが、成功しています。一方②の方法では現在の技術では困難であり、研究の進歩が必要です。今後も研究を継続し、移植可能な肺再生につなげていきたいと考えています。