Mesh-culture system of lung epithelial cells from human undifferentiated cells for future application of infectious disease modelling

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H02925
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: Kyoto University
• Principal Investigator: Ito Isao 京都大学, 医学研究科, 准教授 (40447975)
• Co-Investigator(Kenkyū-buntansha): 今井 晶 (松島晶) 京都大学, 医学研究科, 特定助教 (40828943) ; 今井 誠一郎 京都大学, 医学研究科, 特定助教 (90572610) ; オケヨ ケネディオモンディ 京都大学, 医生物学研究所, 講師 (10634652)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 肺胞上皮細胞 / SP-C / マイクロメッシュ / 気液界面培養 / 未分化細胞 / 血管内皮細胞

Outline of Final Research Achievements

For efficient development and increase of human lung epithelial cells, we adjusted the feeder cells and modified elements of culture medium. As the reslut, we succeeded in improvement of duplication speed of the cells. Air-liquid interface culture enabled the cells express epithelial tight junction markers and surfactant protein-C ans the marker of type II pneumocytes. As the next step, we aimed to establish the two-sided culture system using micromesh-based thin embrane. The membrane with thickness of 1-3 micrometer was established. On the sheed we were able to culture human lung endothelial cells as well. We are now continuing to establish two-dsided culture system.

Free Research Field

呼吸器内科学

Academic Significance and Societal Importance of the Research Achievements

薄型人工膜を用いた上皮細胞と血管内皮細胞の二層培養を成立させることが出来れば、従来の単細胞培養よりも、はるかに生体内を模写したモデルが確立できる。肺の研究のみならず、他臓器の研究へも応用範囲は広い。また、生理的反応や病態の理解に加え、創薬スクリーニングにも用いられる可能性が広がる。