Comprehensive study on establishment of pulp regenerative therapy based on the scientific evidences

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H03117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Niigata University
• Principal Investigator: Noiri Yuichiro 新潟大学, 医歯学系, 教授 (50218286)
• Co-Investigator(Kenkyū-buntansha): 大倉 直人 新潟大学, 医歯学総合病院, 助教 (00547573) ; 松崎 英津子 福岡歯科大学, 口腔歯学部, 教授 (20432924) ; 竹中 彰治 新潟大学, 医歯学系, 准教授 (50313549) ; 朝日 陽子 大阪大学, 大学院歯学研究科, 助教 (50456943) ; 枝並 直樹 新潟大学, 医歯学系, 助教 (80804567)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 歯髄復元 / 動物モデル / SCAP / S1P / 再生歯内療法

Outline of Final Research Achievements

We have developed a rat model of Regenerative Endodontic Procedure (REP) for each root growth process and elucidated the involvement of several MSCs in the early stage of healing based on the localization of Mesenchymal Stem Cells (MSCs) and M2 macrophages (Mφ), which are involved in healing. MSCs are involved in the early stages of healing. The R1 signal of sphingosine 1-phosphate (S1P), an effect modifier of the above, is involved in the calcification of the matrix, i.e., dentin formation, and the number of specific MSCs gradually decreases at the root apex with root growth, causing delayed healing and reduced regenerated pulp tissue after REP, which affects healing morphology. This is the first time that the number of MSCs in the root apex has been investigated.

Free Research Field

歯科保存学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、成熟永久歯の再生歯内療法を成功裡に導く為の科学的根拠となるため、同療法を臨床導入する機運が加速する意味では、医学的価値が高い成果である。また、本研究は、日本で年間500万件以上実施されているにも拘らず、根尖病変発症率が50％以上である現行の根管治療を補完し、歯を不死化する再生療法の確立が最終ゴールであるため、学術的にも社会的にも意義深い成果である。