2023 Fiscal Year Final Research Report
Periodontal tissue regenerative medicine through LAP-dependent tissue remodeling
| Project/Area Number |
21H03118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 57030:Conservative dentistry-related
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
北村 正博 大阪大学, 大学院歯学研究科, 准教授 (10243247)
村上 伸也 大阪大学, 大学院歯学研究科, 教授 (70239490)
岩山 智明 大阪大学, 大学院歯学研究科, 助教 (80757865)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 歯周組織再生 / 脂肪組織由来多系統前駆細胞 / efferycytosis |
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| Outline of Final Research Achievements |
In this study, we investigated the effects of allogeneic transplantation of adipose tissue-derived multilineage progenitor cells (ADMPC) on periodontal tissue regeneration, and the impact of apoptosis in transplanted cells and host apoptotic processing mechanisms on these effects. ADMPCs were transplanted into a rat periodontal tissue defect model, resulting in significant periodontal tissue regeneration. However, the transplanted ADMPCs underwent apoptosis within 14 days post-transplantation. It was found that the transplantation of apoptosis-resistant ADMPCs significantly reduced tissue regeneration effects compared to the control group. Furthermore, in vitro analysis revealed that the macrophages underwent efferocytosis of apoptosis-induced ADMPCs, which altered their phenotype to a reparative one.
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| Free Research Field |
歯周病学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題では、脂肪組織由来間葉系幹細胞の同種移植による歯周組織再生効果と、同効果における分子機序の一つとして細胞のアポトーシスおよび宿主によるefferocytosisの関与が明らかとなった。この結果は、間葉系幹細胞移植治療による抗炎症効果のみならず、組織再生に対しても宿主のアポトーシス細胞処理機構が重要であることを示唆するとともに、幹細胞移植による歯周組織再生療法の開発を推進するための基盤情報となる。
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