2023 Fiscal Year Final Research Report

Intervertebral disc regenerative therapy using bone marrow aspirate concentrate combined with in situ forming bioresorbable ultra-purified alginate gel

Research Project

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Project/Area Number	21H03313
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 59020:Sports sciences-related
Research Institution	Hokkaido University
Principal Investigator	Sudo Hideki 北海道大学, 医学研究院, 特任教授 (30374367)
Co-Investigator(Kenkyū-buntansha)	山田勝久北海道大学, 大学病院, 助教 (20771893) 岩田玲北海道大学, 大学病院, その他 (10374373)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	椎間板再生 / 自家骨髄濃縮液
Outline of Final Research Achievements	The regenerative ability of intervertebral discs (IVDs) is restricted due to the poor nutritional supply. We have demonstrated the regenerative effect of implantation of bone marrow aspirate concentrate (BMAC) combined with ultra-purified alginate gel (UPAL gel) for degenerated IVDs in a rabbit model. The purpose of this study is to assess the effectiveness of the implantation of BMAC combined with UPAL gel after discectomy in beagle dogs as a large animal model. Implantation of the BMAC combined with UPAL gel suppresses IVD degeneration after discectomy. In addition, type II collagen positive cells were significantly higher in the BMAC group, suggesting that the implantation of BMAC combined with UPAL gel promoted spontaneous IVD repair. These results support that BMAC combined with UPAL gel implantation after discectomy may be applied in clinical settings as one-step cell-based regenerative therapy for IVD herniation.
Free Research Field	整形外科
Academic Significance and Societal Importance of the Research Achievements	生体内における椎間板の再生能力は著しく低く、椎間板ヘルニアに対するヘルニア摘出術は術後変性を来す要因となる。我々はこれまでに家兎椎間板変性モデルを対象に、アルギン酸を基盤とした高純度硬化性ゲルと骨髄濃縮液（BMAC）の併用による組織再生効果と力学的安定性を示してきた。本研究では、臨床応用を見据えた橋渡し研究としての性能評価試験を行なった。細胞培養操作を伴う幹細胞移植に比べて一期的処置が可能な本法は、生体侵襲やコスト軽減効果が高く、実臨床における新たな椎間板再生治療法としての有用性は高いと言える。本研究における橋渡し研究の成果が示されたことから今後の臨床への応用が期待される。