2023 Fiscal Year Final Research Report
Establishment of a research platform for individual differences in radiation exposure effects using induced pluripotent stem cells and advanced microscopy
| Project/Area Number |
21H03599
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 63020:Radiation influence-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 放射線被ばく影響評価 / ラマン散乱スペクトル計測 / iPS細胞 |
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| Outline of Final Research Achievements |
This study aimed to establish a technical basis for investigating individual differences in radiation exposure effects on iPS cells (and differentiated cells) using advanced optical imaging technology. First, we developed a technique to evaluate the effect of radiation exposure on DNA structure and metabolic capacity of reactive oxygen species (ROS) by applying a single molecule measurement method. Second, based on Raman scattering spectroscopy, we established a technique to evaluate the differences in human induced pluripotent stem cells (iPS cells) after γ-ray irradiation. Especially, the latter technique exhibited a correlation between radiation exposure tolerance and Raman spectra of human iPS cells of different origins (different races), indicating the practicality and effectiveness of this method.
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| Free Research Field |
生物物理学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、今後の放射線被ばく影響の個人差調査を促進する可能性がある。ラマン散乱スペクトル計測には、トランスクリプトミクス等の生化学計測と比較して、一切の化学的/生物学的調整を必要としないため、非常に迅速にデータを収集できるからだ。一方で、ラマン散乱スペクトルが放射線被ばく耐性を表現できると断定するためには、実験サンプル数が少なく、また、その生物学的根拠も明らかになっていない。今後、計測実例を増やし、また、トランスクリプトミクスやゲノム情報との相関を調べていくことで、将来的には細胞に光を当てるだけで放射線被ばく耐性を推定できるようになると期待される。
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