Molecular dissection of matorisome in a high-regenerative capability animal, salamanders

2023 Fiscal Year Final Research Report

• Project/Area Number: 21H03829
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Suzuki Ken-ichi 基礎生物学研究所, 超階層生物学センター, 特任准教授 (90363043)
• Co-Investigator(Kenkyū-buntansha): 遠藤 哲也 愛知学院大学, 教養部, 准教授 (90399816)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 器官再生 / 両生類 / 四肢再生 / マトリソーム / ゲノム / オミクス解析

Outline of Final Research Achievements

It is believed that regenerating organs contain matrisomes, which are composed of "extracellular matrix" and "secretory growth factors" that ensure cell dedifferentiation and stem cell proliferation. In this study, we performed comparative omics analysis of the matrisomes of newt and frog limb regenerating buds. We knocked out matrisome factors specific to newt regeneration and analyzed their functions in limb regeneration. In addition, we conducted a genome project with the support of the Platform for Advanced Genome Science (PAGS) to improve the protein database required for the advancement of newt matrisome proteomics. In addition, we experimented with transplanting decellularized matrices to induce mouse limb regeneration and evaluated the results.

Free Research Field

再生生物学

Academic Significance and Societal Importance of the Research Achievements

本申請課題によって得られる成果は、以下のような先進的な生体材料の開発に繋がることが期待できる。（１）創傷治癒や器官再生を促進する有用な治療用生体材料の開発。（２）より生体に近い機能と構造を持つオルガノイドの創生技術の開発。（３）幹細胞を生体に近い状態で大量に産生可能な培養技術の開発。
生体材料工学に新機軸を打ち出し、イモリの高い器官再生能力についてマトリソームという新しい視点から解明を行う本申請課題は、これまでに前例のないユニークな研究例となることが期待できる。