2024 Fiscal Year Final Research Report
Biomedical application of fish collagen: Development of the bone-mimetic collagen-fibril coating on the titan-made bone implant
| Project/Area Number |
21H04731
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAGI Yasuaki 北海道大学, 水産科学研究院, 教授 (10212002)
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| Co-Investigator(Kenkyū-buntansha) |
浦 和寛 北海道大学, 水産科学研究院, 准教授 (90360940)
東藤 正浩 北海道大学, 工学研究院, 教授 (10314402)
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| Project Period (FY) |
2021-04-05 – 2025-03-31
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| Keywords | コラーゲン原線維コーティング / 再生医療チタン材料 / 表面加工 / 骨誘導 / 水産廃棄物利用 |
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| Outline of Final Research Achievements |
Leveraging the high fibrillogenesis potential of sturgeon swim bladder-derived collagen, as identified by our research group, we successfully fabricated a bone-mimetic material that replicates the hierarchical structure of natural bone, depositing hydroxyapatite crystals into highly oriented collagen fibrils using a biomimetic mineralization approach. Subsequently, we developed a novel technique for imparting this bone-mimetic architecture onto the surface of titanium alloys commonly employed in orthopedic implants such as artificial joints. Furthermore, we demonstrated that non-mineralized, highly oriented collagen fibrils effectively promote the osteogenic differentiation of human mesenchymal stem cells. Future work will involve a more detailed investigation of cellular responses to the bone-mimetic architecture and transplantation studies using animal models to evaluate in vivo performance.
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| Free Research Field |
魚類硬組織生理学
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| Academic Significance and Societal Importance of the Research Achievements |
本成果の学術的意義は，非石灰化高配向性コラーゲン原線維が間葉系幹細胞から骨芽細胞への分化の初期（分化運命決定）および中期を強く促進することを証明した点にある。このことは，細胞足場材料のコラーゲン原線維の形態（太さや配向性）が幹細胞の分化に強く影響することを示している。また，真に生体骨の構造を模倣した「骨ミメティック材料」の創出と，これをチタン合金にコートする骨ミメティックコート技術の開発の成功は，将来の人工骨やインプラント表面改質技術への発展が期待できるため，社会的意義が高い。
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