2021 Fiscal Year Annual Research Report
Studies on Nanometer-sized Artificial Basement Membranes for Cell Compartmentalization in Three-dimensional Tissues
| Project/Area Number |
21J12833
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Zeng Jinfeng 大阪大学, 工学研究科, 特別研究員(PD)
|
|---|
| Project Period (FY) |
2021-04-28 – 2023-03-31
|
| Keywords | Artificial BMs / Compartmentalization / ECM proteins / Cell migration / Tissue engineering |
|---|
| Outline of Annual Research Achievements |
My research topic is to construct artificial basement membranes (A-BMs) to keep cell and tissue compartmentalization in vitro.
In our previous study, type IV collagen (Col-IV) and laminin (LM) assembled nanofilm was demonstrated to prevent endothelial cells migration and keep cells compartmentalized structure.
This year, compared with other ECM proteins, the advantage of Col-IV and LM as the main components to prepare A-BMs was evaluated. Learned from previous study, polyelectrolyte nanofilm has a higher density and is more stable during cell culture, which will exhibit different abilities to prevent cell migration. Thus, the structure and bio-functions of polyelectrolyte assembled nanofilms were compared with Col-IV/LM nanofilms.
In our previous study, type IV collagen (Col-IV) and laminin (LM) assembled nanofilm was demonstrated to prevent endothelial cells migration and keep cells compartmentalized structure.
This year, compared with other ECM proteins, the advantage of Col-IV and LM as the main components to prepare A-BMs was evaluated. Learned from previous study, polyelectrolyte nanofilm has a higher density and is more stable during cell culture, which will exhibit different abilities to prevent cell migration. Thus, the structure and bio-functions of polyelectrolyte assembled nanofilms were compared with Col-IV/LM nanofilms.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The study went well basically, following my research plan. Until now, the advantage of col-IV and LM as the main components was confirmed. Transglutaminase crosslinked nanofilms extended the time of cell separation due to the enhanced stability. And then, based on polyelectrolyte assembled nanofilms, cell compartmentalization time can be extended again to 2 weeks from 5 days. Considering the secretion of Col-IV and LM by cells and re-constructure of natural basement membranes around 15 days. The ability to prevent cell migration within 2 weeks, the founding will give powerful support to constructing organized 3D tissue in vitro.
I planned to print patterned nanofilms by inkjet printing. However, the use and proper printing parameters took much longer time than I expected.
|
| Strategy for Future Research Activity |
This year, firstly, I am going to summarize current results and publish at least one paper.
And then, the construction of patterned A-BMs in 3D tissues will be further studied. Inkjet printing onto living cell monolayer is still facing huge challenges due to the printing force. Cell detachment happened, inducing the instability between cells. This issue should be deliberated and solved this year. At the same time, cell printing using inkjet printing has low cell viability. Single-cell encapsulation by ECM proteins will be considered to protect cells, avoiding shear force during printing and increasing cell viability.
After the successful fabrication of patterned nanofilms between cells, the structure and biofunctions of tailored A-BMs will be evaluated to construct organized 3D tissues.
|
