2022 Fiscal Year Annual Research Report

Studies on Nanometer-sized Artificial Basement Membranes for Cell Compartmentalization in Three-dimensional Tissues

Research Project

Project/Area Number	21J12833
Research Institution	Osaka University
Principal Investigator	Zeng Jinfeng 大阪大学, 工学研究科, 特別研究員(PD)
Project Period (FY)	2021-04-28 – 2023-03-31
Keywords	Artificial BMs / Compartmentalization / cation-dipole / surface charge / organized 3D tissue
Outline of Annual Research Achievements	In this year, we evaluated the barrier effects of poly-L-lysine/dextran nanofilms (PLL/D NFs) constructed via the cation-dipole interaction to maintain compartmentalized cell co-culture of NHDF and HUVEC. PLL/D NFs exhibited comparable abilities to Col-IV/LM NFs in terms of cell adhesion, proliferation, and differentiation. However, it is worth noting that PLL/D NFs maintained compartmentalized cell co-culture for over two weeks, much longer than assembled nanofilms based on ECM proteins. Furthermore, the secretion of type IV collagen was observed under the confluent HUVEC monolayer, suggesting the re-construction of natural BMs in the in-vitro cultured tissues. The efficient barrier effects of PLL/D NFs provided a powerful support for constructing organized 3D tissues in vitro.
Research Progress Status	令和4年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	令和4年度が最終年度であるため、記入しない。