2021 Fiscal Year Annual Research Report
ラマン・蛍光イメージングフローサイトメトリーとセルソーティング
| Project/Area Number |
21J15001
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| Research Institution | Osaka University |
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Principal Investigator |
LINDLEY Matthew Mark 大阪大学, 工学研究科, 特任研究員
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| Project Period (FY) |
2021-04-28 – 2022-03-31
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| Keywords | Raman / Cell Sorting / Label-free |
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| Outline of Annual Research Achievements |
The goal of this project was to establish simultaneous Raman & fluorescence cell sorting based & imaging flow cytometry. This required combined development in three fields: nonlinear spectroscopy, microfluidics, and real-time data processing and decision making. At the time of this report, I have achieved cell sorting based on analysis of broadband, fingerprint region Raman spectra at throughputs of up to ~50 cells/s and purities up to 93%. This work required constructing the cell sorter, a proof of concept sorting using the Raman signal from polymer microbeads, and sorting cells based on the Raman signal from their polysaccharide and carotenoid content. A manuscript based on the results is currently under review.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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