Fundamental Analysis of Novel Fruit and Vegetable Severe Allergen Component GRP

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K02118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 08030:Family and consumer sciences, and culture and living-related
• Research Institution: Kyoto Women's University
• Principal Investigator: Momma Keiko 京都女子大学, 家政学部, 教授 (70397561)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 食物アレルギー / 重症果物アレルゲン / GRP / モノクローナル抗体

Outline of Final Research Achievements

Gibberellin-regulated protein (GRP), a severe allergen of peach, is highly conserved among plants, and allergic reactions to GRP as a causative agent have been reported in fruits and vegetables other than peach. Furthermore, GRP has been reported to be present in cypress pollen, and its relationship to allergy has been emphasized. In this study, we purified GRP from various fruits, vegetables, and pollens and measured the GRP content in fruits and vegetables using the cross-reactivity of anti-peach GRP monoclonal antibodies, and verified their allergenicity. Furthermore, monoclonal antibodies against cedar pollen GRPs were generated.

Free Research Field

食品科学

Academic Significance and Societal Importance of the Research Achievements

果物アレルギーは、若年以降に発症することが多く完治しにくい。また１割程度は重篤な症状を示す。モモ重症アレルゲンであるGibberellin-regulated protein (GRP)は植物間で保存されていることから、他の果物・野菜における存在量を明らかにすることで、アレルギー患者のQOLの向上に寄与することができる。また純化したGRPは患者のアレルゲンの責任抗原の特定に役立てられる。さらに得られた抗体を用いて花粉症との関連について研究を進める予定である。