2023 Fiscal Year Final Research Report
Mechanical evaluation for articular cartilage based on molecular and crystal scaled structural analysis by Raman imaging
| Project/Area Number |
21K03744
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 18010:Mechanics of materials and materials-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 生物・生体工学 / 生体分子 / 医療・福祉 / 関節軟骨 / ラマン分光 |
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| Outline of Final Research Achievements |
The number of people suffering from knee osteoarthritis is increasing with the aging of the population, and there is an urgent need to investigate the cause and establish a treatment method. The purpose of this study was to quantitatively evaluate the progression of knee osteoarthritis by analyzing changes in the internal structure of cartilage due to stress. Raman spectroscopy is a method that uses the Raman scattering that is emitted when a material is exposed to visible light and outputs it as a spectrum called Raman shift. In the experiment, it was confirmed that the peak of the collagen component, which is a cartilage component, shifted before and after compression. Furthermore, when we compared the Raman spectra before and after applying a compressive load with and without degeneration treatment, we confirmed that there was a difference in the change in the peak position, indicating the possibility of obtaining the degeneration state of cartilage.
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| Free Research Field |
生体機械工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究を通じて，ラマン分光法と力学負荷を組み合わせることで，通常軟骨と変性軟骨を区別できることを示すことができた．また，応力緩和中の軟骨表面組織の状態をラマンスペクトルから読み取れることが示された．これらの結果より，ラマン分光法と力学負荷を組み合わせることで，OA 初期段階における軟骨の異常診断，プローブ等の臨床応用にも有効となる可能性が示された．このことは，低侵襲かつ定量的な関節軟骨機能評価手法によって，初期関節症の早期発見の可能性を示すものであり，本手法に基づく医療診断装置が実現できれば，患者への負担も大幅に抑制できるものである．
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