Construction of rapid enzyme linked immunosorbent assay for infectious disease testing using electric field mixing (EFM)

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K03845
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 18040:Machine elements and tribology-related
• Research Institution: Akita Industrial Technology Center
• Principal Investigator: Nakamura Ryuta 秋田県産業技術センター, 先進プロセス開発部, 主任研究員 (00634213)
• Co-Investigator(Kenkyū-buntansha): 赤上 陽一 秋田県産業技術センター, 企画事業部, 専門員 (00373217) ; 大久保 義真 秋田県産業技術センター, 先進プロセス開発部, 研究員 (30826532)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 撹拌 / 電界 / 電界撹拌 / 抗原抗体反応 / ELISA / 酵素基質反応 / 光質センサ

Outline of Final Research Achievements

In this study, we selected a substrate for the ELISA method that can be stirred by electric field. By using a glass plate with little variation in absorbance, electric field mixing was enabled, and by introducing electric field mixing into the antigen-antibody reaction and color development process, it was confirmed that the antigen-antibody reaction and enzyme-substrate reaction could be accelerated. Furthermore, we confirmed that even a small amount of liquid can be evaluated by measuring saturation using a light quality sensor instead of the current absorbance measurement. It was also confirmed that the saturation was improved and the variation was smaller compared to the static method.

Free Research Field

機械工学

Academic Significance and Societal Importance of the Research Achievements

本研究にて、電界撹拌が可能な最適な基板を選定し、E L I S A法へ電界撹拌技術を導入可能とした。これにより、従来長時間を要する定量検査であるE L I S A法の迅速化と使用検体量の削減可能性を提案できた。研究開始時には猛威を奮っていたCIVID-19をはじめ、インフルエンザ、風疹等様々な感染症抗体検査へ応用可能な日本発の技術で学術的かつ社会的貢献を実現可能だと考える。