Development of metabolic control technology based on nanosecond pulsed electric field using metabolomics

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K04116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 21040:Control and system engineering-related
• Research Institution: Sasebo National College of Technology
• Principal Investigator: Koshimura Masahiro 佐世保工業高等専門学校, 物質工学科, 准教授 (80610310)
• Co-Investigator(Kenkyū-buntansha): 山崎 隆志 佐世保工業高等専門学校, 物質工学科, 教授 (20270382) ; 猪原 武士 佐世保工業高等専門学校, 電気電子工学科, 准教授 (30634050)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: メタボロミクス / ナノ秒パルス電界 / 出芽酵母 / 代謝

Outline of Final Research Achievements

In this grant, we examined comprehensive analysis of metabolites and gene expression analysis, focusing on the effects of nanosecond pulsed electric fields on the metabolism of budding yeast. It was found that the amount of alcohol produced by budding yeast was not affected by the application of nsPEF. On the other hand, there were differences in intracellular hydrophilic metabolites between the control and treated groups. Furthermore, it was found that there were differences in gene expression among the groups, indicating that nsPEF may be applicable to metabolic control.

Free Research Field

農芸化学

Academic Significance and Societal Importance of the Research Achievements

超短時間で高エネルギーを放出することができるパルスパワー技術を利用したナノ秒パルス電界（nsPEF）は非加熱的に細胞内の状態を変化させることができるため，生物学分野への応用が期待されているが，作用メカニズムについて不明な点が多いことが問題となっている。今回nsPEF印加によって出芽酵母の遺伝子発現および代謝物質に変化が生じることが明らかとなり，nsPEF印加が微生物の代謝制御技術に繋がることが期待される。