2021 Fiscal Year Research-status Report
Development of supercritical carbon dioxide mediated microparticles of juvenile hormone analogues (JHAs) for the elimination of mosquito-borne diseases
| Project/Area Number |
21K04758
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| Research Institution | Fukuoka University |
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Principal Investigator |
シャーミン タンジナ 福岡大学, 工学部, 助教 (00794182)
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| Co-Investigator(Kenkyū-buntansha) |
相田 卓 福岡大学, 工学部, 助教 (00466541)
三島 健司 福岡大学, 工学部, 教授 (40190623)
佐藤 朝光 福岡大学, 薬学部, 准教授 (90369025)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | carbon dioxide / Protein microparticles / supercritical fluids / hormone analogues |
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| Outline of Annual Research Achievements |
In collaboration with my coworkers, we have examined the development of several supercritical carbon dioxide mediated microparticles of JHA hormones using particles from gas saturated solutions (PGSS) process, and the liquid-liquid two-phase separation. Insect growth regulator pyriproxyfen has been successfully encapsulated in poly-(e-caprolactone) (MW:10000~80000 g/mol). The effect of process conditions, i.e., pre-expansion temperature and pressure, and polymer mass ratio has been analyzed. Particles were evaluated for particle shape and size, encapsulation efficiency (using HPLC), Fourier transform infrared (FTIR) spectroscopy to confirm entrapment. Encapsulated particles with average particle sizes of 73.6 ± 2.3 micro-meter were obtained with encapsulation efficiency up to 78 ± 9.4 %. The efficiency of encapsulation was increased by conditions that favored a fast solidification of the polymer shell, including high pre-expansion pressures, reaching efficiencies up to 88.5 ± 1.10 %. Finally, the controlled release of the active material (pyriproxyfen) was obtained for 150 days to evaluate the adult emergence inhibition (EI). Finally, the release of the active material was obtained at a controlled rate with average 38.4 ± 0.56 ppb/day for 150 days which is to evaluate the adult emergence inhibition (EI). Our results showed an excellent potential of the Controlled release systems for in vitro and field application. A laboratory evaluation of mosquito emergence inhibition is under way to confirm the effectiveness of mosquito larvicidal activity.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
This fund provided enough financial support to purchase all equipment's and chemical as required for the research made the smooth execution of this research project.
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| Strategy for Future Research Activity |
Dissolution assay has been successfully carried out for 150 days to evaluate the release rate in daily exchanged water treated with JHAs microparticles. At present, a bioassay on larval mortality (laboratory scale) is carrying out in the Faculty of Pharmaceutical Sciences, Fukuoka University using 4th instar larvae of Aedes albopictus. By this time we will get enough data to publish the result of this investigation.
In parallels, we will develop a digital Recognition system of liquid-liquid two-phase separation by digital image processing for the cost reduction of the entire process. The products of a chemical reaction such as a drug or an organic solvent are often found in an impure state such as a mixture of two or more substances and a separation process is essential for the recovery of the primary product from raw materials. The separation process is so important that this accounts for more than 70% of the capital in a typical chemical plant. In order to reduce the operating cost such as labor costs, it is desired to develop an automated method for the recognition of phase separation by artificial intelligence or image recognition technology. Addressing this issue, we desired to develop a simple recognition system that could be applicable to any aqueous-organic or aqueous-aqueous two-phase systems regardless of restricted with any specific conditions. The result of this investigation is also publish under this funds support.
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Research Products
(3 results)
