Construction of simple biocatalyst for efficient bioconversion

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K04792
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Hiroshima University
• Principal Investigator: Tajima Takahisa 広島大学, 統合生命科学研究科(先), 准教授 (80571116)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 酵素触媒 / 低温菌 / 熱処理

Outline of Final Research Achievements

In this study, we constructed a simple biocatalyst for conversion of valuable chemical and analyzed the reaction field for sustained conversion.
In the construction of the biocatalyst, a simple biocatalyst for conversion of ferulic acid to vanillin was constructed, and a method and tool for improving the expression of low activity enzymes in a two-step enzymatic reaction were developed. In the reaction field analysis, we studied the effect of heat treatment on the reaction field (cells) in the simple biocatalyst. Heat treatment is performed at a moderate temperature to suppress the metabolic activity of the host and improve the membrane permeability of the substrate, but there are concerns about the effects of heat treatment. An evaluation system using fluorescent proteins was constructed, and quantitative evaluation of protein leakage from the reaction field by fluorescence intensity was performed.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

本研究はバイオによる有用化学品の効率的な生産技術に関わるシンプル酵素触媒の構築を目的としたものである。変換酵素を抽出・精製せずに用いることが可能な本触媒は手間やコストをかけずに効率的な酵素変換を実現できるものとして期待される。本研究により持続的に変換するための触媒構築や熱処理方法を見出され、本触媒の工業的利用が期待される。